BMRB Entry 5203
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5203
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Title: 1H, 13C, 15N chemical shift assignments for cysteine-rich domain of KSR PubMed: 11786023
Deposition date: 2001-11-06 Original release date: 2002-05-07
Authors: Zhou, Ming; Horita, David; Waugh, David; Byrd, R.; Morrison, Deborah
Citation: Zhou, Ming; Horita, David; Waugh, David; Byrd, R.; Morrison, Deborah. "Solution Structure and Functional Analysis of the Cysteine-rich C1 Domain of Kinase Suppressor of Ras (KSR) " J. Mol. Biol. 315, 435-446 (2002).
Assembly members:
Kinase suppressor of Ras, polymer, 49 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Kinase suppressor of Ras: GSVTHRFSTKSWLSQVCNVC
QKSMIFGVKCKHCRLKCHNK
CTKEAPACR
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 270 |
| 13C chemical shifts | 138 |
| 15N chemical shifts | 44 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | cysteine-rich C1 domain of KSR | 1 |
| 2 | Zinc(II) ion, I | 2 |
| 3 | Zinc(II) ion, II | 2 |
Entities:
Entity 1, cysteine-rich C1 domain of KSR 49 residues - Formula weight is not available
| 1 | GLY | SER | VAL | THR | HIS | ARG | PHE | SER | THR | LYS | ||||
| 2 | SER | TRP | LEU | SER | GLN | VAL | CYS | ASN | VAL | CYS | ||||
| 3 | GLN | LYS | SER | MET | ILE | PHE | GLY | VAL | LYS | CYS | ||||
| 4 | LYS | HIS | CYS | ARG | LEU | LYS | CYS | HIS | ASN | LYS | ||||
| 5 | CYS | THR | LYS | GLU | ALA | PRO | ALA | CYS | ARG |
Entity 2, Zinc(II) ion, I - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: Kinase suppressor of Ras, [U-15N; U-13C], 1.0 mM; ZINC (II) ION 2.0 mM
Ex-cond_1: pH: 6.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HSQC | sample_1 | not available | Ex-cond_1 |
| NOESY-HSQC | sample_1 | not available | Ex-cond_1 |
| CBCACONH | sample_1 | not available | Ex-cond_1 |
| HNCACB | sample_1 | not available | Ex-cond_1 |
| TOCSY-HSQC | sample_1 | not available | Ex-cond_1 |
| 3dCN-NOESY | sample_1 | not available | Ex-cond_1 |
| HCCH TOCSY | sample_1 | not available | Ex-cond_1 |
Software:
Ansig v3.3 -
NMR spectrometers:
- Varian UnityPlus 500 MHz
- Varian UnityPlus 600 MHz
Related Database Links:
| SWISS-PROT | Q61097 |
| REF | NP_038599 |
| GenBank | EDL15602 EDL15601 ABK42251 AAI68386 AAC52382 |
| EMBL | CAI24047 CAI24046 CAA57288 |
| DBJ | BAE23398 |
| PDB |
Download simulated HSQC data in one of the following formats:
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or all simulated shifts
SPARKY: Backbone
or all simulated shifts