BMRB Entry 5256

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5256

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Solution Structure of the Unmodified Anticodon Stem-loop from E. coli tRNA(Phe) PubMed: 12079344

Deposition date: 2002-01-11 Original release date: 2002-09-10

Authors: Cabello-Villegas, J.; Winkler, M.; Nikonowicz, E.

Citation: Cabello-Villegas, J.; Winkler, M.; Nikonowicz, E.. "Solution Conformations of Unmodified and A(37)N(6)-dimethylallyl Modified Anticodon Stem-loops of Escherichia coli tRNA (Phe)" J. Mol. Biol. 319, 1015-1034 (2002).

Assembly members:
anticodon stem-loop tRNA(Phe), polymer, 17 residues, Formula weight is not available

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source: Production method: enzymatic semisynthesis

Entity Sequences (FASTA):
anticodon stem-loop tRNA(Phe): GGGGAUUGAAAAUCCCC

Data sets:

assigned_chemical_shifts
- chemical_shift_1
- chemical_shift_2

Data type	Count
13C chemical shifts	110
15N chemical shifts	16
31P chemical shifts	17

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	anticodon stem-loop of tRNA(PHE)	1

Entities:

Entity 1, anticodon stem-loop of tRNA(PHE) 17 residues - Formula weight is not available

1

G

A

U

G

A

2

A

U

C

Samples:

sample_1: anticodon stem-loop tRNA(Phe), [U-98% 15N], 2 mM; NaCl 10.0 mM; KPi 10.0 mM

sample_2: anticodon stem-loop tRNA(Phe), [U-95% 13C], 1.0 – 2.3 mM; NaCl 10.0 mM; KPi 10.0 mM

sample_cond_1: ionic strength: 20 mM; pH: 6.8; pressure: 1 atm; temperature: 285 K

sample_cond_2: ionic strength: 20 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	not available	not available	not available
3D 13C-separated NOESY	not available	not available	not available
DQF-COSY	not available	not available	not available
HNN-COSY	not available	not available	not available
P31-1H HetCor	not available	not available	not available

Software:

X-PLOR v3.861 & 3.1 - refinement, structure solution

FELIX v98 - data analysis

NMR spectrometers:

Bruker AMX 500 MHz