BMRB Entry 5262
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5262
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Title: Backbone 1H and 15N Chemical Shift Assignements for the first fibronectin type II module of MMP-2 (col-1) PubMed: 11928808
Deposition date: 2002-01-20 Original release date: 2002-06-13
Authors: Gehrmann, Marion; Briknarova, Klara; Banyai, Laszlo; Patthy, Laszlo; Llinas, Miguel
Citation: Gehrmann, Marion; Briknarova, Klara; Banyai, Laszlo; Patthy, Laszlo; Llinas, Miguel. "The Col-1 Module of Human Matrix Metalloproteinase-2 (MMP-2): Structural/Functional Relatedness between Gelatin-binding Fibronectin Type II Modules and Lysine-binding Kringle Domains" Biol. Chem. 383, 137-148 (2002).
Assembly members:
the first fibronectin type II module of human matrix metalloproteinase, polymer, 60 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
the first fibronectin type II module of human matrix metalloproteinase: RIPVKYGNADGEYCKFPFLF
NGKEYNSCTDTGRSDGFLWC
STTYNFEKDGKYGFCPHEAL
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 62 |
| 1H chemical shifts | 397 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | col-1 | 1 |
Entities:
Entity 1, col-1 60 residues - Formula weight is not available
| 1 | ARG | ILE | PRO | VAL | LYS | TYR | GLY | ASN | ALA | ASP | |
| 2 | GLY | GLU | TYR | CYS | LYS | PHE | PRO | PHE | LEU | PHE | |
| 3 | ASN | GLY | LYS | GLU | TYR | ASN | SER | CYS | THR | ASP | |
| 4 | THR | GLY | ARG | SER | ASP | GLY | PHE | LEU | TRP | CYS | |
| 5 | SER | THR | THR | TYR | ASN | PHE | GLU | LYS | ASP | GLY | |
| 6 | LYS | TYR | GLY | PHE | CYS | PRO | HIS | GLU | ALA | LEU |
Samples:
sample_1: the first fibronectin type II module of human matrix metalloproteinase 0.5 mM; H2O 90%; acetone, [U-2H], 10%
sample_2: the first fibronectin type II module of human matrix metalloproteinase 0.5 mM
sample_3: the first fibronectin type II module of human matrix metalloproteinase, [U-15N], 0.7 mM
condition_1: pH*: 7.3; temperature: 298 K
condition_2: pH*: 6.0; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D COSY | not available | not available | not available |
| 2D TOCSY | not available | not available | not available |
| 2D NOESY | not available | not available | not available |
| 2D 1H-15N HSQC | not available | not available | not available |
| 3D HNHA | not available | not available | not available |
| 3D HNHB | not available | not available | not available |
| 3D 15N-edited TOCSY | not available | not available | not available |
| 3D 15N-edited NOESY | not available | not available | not available |
Software:
XWINNMR v2.0 - experiment acquisition
FELIX v98 - data processing and assignment
XPLOR v3.851 - structure calculation
NMR spectrometers:
- Bruker AvanceDMX 500 MHz
Related Database Links:
| PDB |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts