BMRB Entry 5278

Name: PEMV-1 P1-P2 Frameshifting Pseudoknot Regularized Average Structure
Published: 2003-02-20

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PDB ID: 1kpz
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5278
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: PEMV-1 P1-P2 Frameshifting Pseudoknot Regularized Average Structure PubMed: 12225754

Deposition date: 2002-02-06 Original release date: 2003-02-20

Authors: Nixon, P.; Giedroc, D.

Citation: Nixon, P.; Rangan, A.; Kim, Y-G.; Rich, A.; Hoffman, D.; Hennig, M.; Giedroc, D.. "Solution Structure of a Luteoviral P1-P2 frameshifting mRNA Pseudoknot" J. Mol. Biol. 322, 621-633 (2002).

Assembly members:
Pea Enation Mosaic Virus Pseudoknot, polymer, 33 residues, Formula weight is not available

Natural source: Common Name: Pea enation mosaic virus Taxonomy ID: 12290 Superkingdom: Viruses Kingdom: not available Genus/species: Enamovirus Pea enation mosaic virus

Experimental source: Production method: enzymatic semisynthesis

Entity Sequences (FASTA):
Pea Enation Mosaic Virus Pseudoknot: GAAUUCCGGUXGACUCCGGA GAAACAAAGUCAA

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
1H chemical shifts	173
13C chemical shifts	36
15N chemical shifts	66

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	P1-P2 frameshifting pseudoknot	1

Entities:

Entity 1, P1-P2 frameshifting pseudoknot 33 residues - Formula weight is not available

1

G

A

U

C

G

U

2

CH

G

A

C

U

C

G

A

3

G

A

C

A

G

U

4

C

A

Samples:

sample_1: Pea Enation Mosaic Virus Pseudoknot 2 mM; KPO4 10 mM; KCl 100 mM; MgCl2 5 mM

sample_2: Pea Enation Mosaic Virus Pseudoknot, [U-95% 15N], 0.2 mM; KPO4 10 mM; KCl 100 mM; MgCl2 5 mM

sample_3: Pea Enation Mosaic Virus Pseudoknot, [U-95% 13C; U-95% 15N], 0.3 mM; KPO4 10 mM; KCl 100 mM; MgCl2 5 mM

sample_cond_1: pH: 6.0; temperature: 283 K; ionic strength: 110 mM; pressure: 1 atm

sample_cond_2: pH: 6.0; temperature: 298 K; ionic strength: 100 mM; pressure: 1 atm

sample_cond_3: pH: 5.8; temperature: 283 K; ionic strength: 100 mM; pressure: 1 atm

Experiments:

Name	Sample	Sample state	Sample conditions
2D NOESY	not available	not available	not available

Software:

X-PLOR v3.851 - structure solution, refinement

NMR spectrometers:

Varian INOVA 600 MHz
Varian INOVA 500 MHz