BMRB Entry 5292
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5292
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Title: 1H Chemical Shift Assignments for TC5b PubMed: 11979279
Deposition date: 2002-02-15 Original release date: 2002-06-13
Authors: Neidigh, Jonathan; Fesinmeyer, R.; Andersen, Niels
Citation: Neidigh, Jonathan; Fesinmeyer, R.; Andersen, Niels. "Designing a 20-residue Protein" Nat. Struct. Biol. 9, 425-430 (2002).
Assembly members:
Trp-cage Construct 5b, polymer, 20 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
Trp-cage Construct 5b: NLYIQWLKDGGPSSGRPPPS
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 276 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TC5b | 1 |
Entities:
Entity 1, TC5b 20 residues - Formula weight is not available
| 1 | ASN | LEU | TYR | ILE | GLN | TRP | LEU | LYS | ASP | GLY | |
| 2 | GLY | PRO | SER | SER | GLY | ARG | PRO | PRO | PRO | SER |
Samples:
sample_1: Trp-cage Construct 5b1.0 – 1.8 mM; phosphate buffer 15 mM; H2O 100%
sample_2: Trp-cage Construct 5b1.0 – 1.8 mM; phosphate buffer 15 mM; Trifluoroethanol 30%; H2O 70%
Aqueous_Buffer: pH: 7; temperature: 282 K
TFE: pH: 7; temperature: 280 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| TOCSY (15-60ms spinlock) | not available | not available | not available |
| NOESY (60-200ms mixing time) | not available | not available | not available |
Software:
xwinnmr - data collection
FELIX v95 - manual peak assignment, processing
NMR spectrometers:
- Bruker DRX 500 MHz
Related Database Links:
| PDB |