BMRB Entry 5317
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5317
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Title: Solution structure of the DNA binding domain of human TRF1 PubMed: 11738049
Deposition date: 2002-03-12 Original release date: 2002-04-08
Authors: Nishikawa, T.; Okamura, H.; Nagadoi, A.; Konig, P.; Rhodes, D.; Nishimura, Y.
Citation: Nishikawa, T.; Okamura, H.; Nagadoi, A.; Konig, P.; Rhodes, D.; Nishimura, Y.. "Solution structure of a telomeric DNA complex of human TRF1" Structure 9, 1237-1251 (2001).
Assembly members:
TRF1, polymer, 69 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
TRF1: TPEKHRARKRQAWLWEEDKN
LRSGVRKYGEGNWSKILLHY
KFNNRTSVMLKDRWRTMKKL
KLISSDSED
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 461 |
| 13C chemical shifts | 196 |
| 15N chemical shifts | 78 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TRF1 | 1 |
Entities:
Entity 1, TRF1 69 residues - Formula weight is not available
| 1 | THR | PRO | GLU | LYS | HIS | ARG | ALA | ARG | LYS | ARG | ||||
| 2 | GLN | ALA | TRP | LEU | TRP | GLU | GLU | ASP | LYS | ASN | ||||
| 3 | LEU | ARG | SER | GLY | VAL | ARG | LYS | TYR | GLY | GLU | ||||
| 4 | GLY | ASN | TRP | SER | LYS | ILE | LEU | LEU | HIS | TYR | ||||
| 5 | LYS | PHE | ASN | ASN | ARG | THR | SER | VAL | MET | LEU | ||||
| 6 | LYS | ASP | ARG | TRP | ARG | THR | MET | LYS | LYS | LEU | ||||
| 7 | LYS | LEU | ILE | SER | SER | ASP | SER | GLU | ASP |
Samples:
sample_1: TRF1, [U-15N; U-13C], 1.5 – 2.5 mM; phosphate buffer 50 mM; NaCl 100 mM; H2O 90%; D2O 10%
sample_2: TRF1, [U-15N; U-13C], 1.5 – 2.5 mM; phosphate buffer 50 mM; NaCl 100 mM; D2O 100%
sample_cond_1: pH: 6.8; temperature: 300 K; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNHA | not available | not available | not available |
| 3D HNHB | not available | not available | not available |
| 3D Sequential assignment protocol | not available | not available | not available |
| 3D 15N-separated NOESY | not available | not available | not available |
| 3D 13C-separated NOESY | not available | not available | not available |
| 2D NOESY | not available | not available | not available |
| 2D TOCSY | not available | not available | not available |
| 2D DQF-COSY | not available | not available | not available |
Software:
EMBOSS v5.0 - refinement
NMR spectrometers:
- Bruker DMX 600 MHz
Related Database Links:
| BMRB | 4210 5361 |
| PDB | |
| DBJ | BAE88046 BAF85218 |
| EMBL | CAA63768 CAH92488 |
| GB | AAB53363 AAB54036 AAB81137 AAC02531 AAH29378 |
| REF | NP_001126467 NP_003209 NP_059523 XP_001083645 XP_001164723 |
| SP | O55036 P54274 |
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