BMRB Entry 5356
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5356
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Title: Backbone and sidechain chemical shift assignments for GS-a3W protein PubMed: 12224922
Deposition date: 2002-04-28 Original release date: 2003-01-06
Authors: Dai, Qing-Hong; Tommos, Cecilia; Fuentes, Ernesto; Dutton, P.; Wand, A.
Citation: Dai, Qing-Hong; Tommos, Cecilia; Fuentes, Ernesto; Dutton, P.; Wand, A.. "Structure of a De Novo Designed Protein Model of Radical Enzymes" J. Am. Chem. Soc. 124, 10952-10953 (2002).
Assembly members:
GS-a3W, polymer, 67 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
GS-a3W: GSRVKALEEKVKALEEKVKA
LGGGGRIEELKKKWEELKKK
IEELGGGGEVKKVEEEVKKL
EEEIKKL
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 515 |
| 13C chemical shifts | 312 |
| 15N chemical shifts | 66 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | GS-a3W protein | 1 |
Entities:
Entity 1, GS-a3W protein 67 residues - Formula weight is not available
| 1 | GLY | SER | ARG | VAL | LYS | ALA | LEU | GLU | GLU | LYS | ||||
| 2 | VAL | LYS | ALA | LEU | GLU | GLU | LYS | VAL | LYS | ALA | ||||
| 3 | LEU | GLY | GLY | GLY | GLY | ARG | ILE | GLU | GLU | LEU | ||||
| 4 | LYS | LYS | LYS | TRP | GLU | GLU | LEU | LYS | LYS | LYS | ||||
| 5 | ILE | GLU | GLU | LEU | GLY | GLY | GLY | GLY | GLU | VAL | ||||
| 6 | LYS | LYS | VAL | GLU | GLU | GLU | VAL | LYS | LYS | LEU | ||||
| 7 | GLU | GLU | GLU | ILE | LYS | LYS | LEU |
Samples:
sample_1: GS-a3W, [U-13C; U-15N], 0.83 mM
sample_2: GS-a3W, [10% 13C], 0.85 mM
Ex-cond_1: pH*: 5.5; temperature: 301.0 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
No software information available
NMR spectrometers:
- Varian Inova 750 MHz
- Varian Inova 500 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts