BMRB Entry 5401
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5401
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Title: Conservation of chemical shift and secondary structure of the PNT/SAM domains from the Ets family of transcription factors PubMed: 12449421
Deposition date: 2002-06-17 Original release date: 2004-08-19
Authors: Mackereth, Cameron; Schaerpf, Manuela; Gentile, Lisa; McIntosh, Lawrence
Citation: Mackereth, Cameron; Scharpf, Manuela; Gentile, Lisa; McIntosh, Lawrence. "Chemical Shift and Secondary Structure Conservation of the PNT/SAM Domains from the ets Family of Transcription Factors" J. Biomol. NMR 24, 71-72 (2002).
Assembly members:
GABPalpha Pointed Domain, polymer, 87 residues, 10339 Da.
Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
GABPalpha Pointed Domain: AALEGYRKEQERLGIPYDPI
HWSTDQVLHWVVWVMKEFSM
TDIDLTTLNISGRELCSLNQ
EDFFQRVPRGEILWSHLELL
RKYVLAS
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 635 |
| 13C chemical shifts | 412 |
| 15N chemical shifts | 99 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | GABPalpha Pointed domain | 1 |
Entities:
Entity 1, GABPalpha Pointed domain 87 residues - 10339 Da.
| 1 | ALA | ALA | LEU | GLU | GLY | TYR | ARG | LYS | GLU | GLN | ||||
| 2 | GLU | ARG | LEU | GLY | ILE | PRO | TYR | ASP | PRO | ILE | ||||
| 3 | HIS | TRP | SER | THR | ASP | GLN | VAL | LEU | HIS | TRP | ||||
| 4 | VAL | VAL | TRP | VAL | MET | LYS | GLU | PHE | SER | MET | ||||
| 5 | THR | ASP | ILE | ASP | LEU | THR | THR | LEU | ASN | ILE | ||||
| 6 | SER | GLY | ARG | GLU | LEU | CYS | SER | LEU | ASN | GLN | ||||
| 7 | GLU | ASP | PHE | PHE | GLN | ARG | VAL | PRO | ARG | GLY | ||||
| 8 | GLU | ILE | LEU | TRP | SER | HIS | LEU | GLU | LEU | LEU | ||||
| 9 | ARG | LYS | TYR | VAL | LEU | ALA | SER |
Samples:
15N_GABPa_PNT: GABPalpha Pointed Domain, [U-99% 15N], 0.5 – 2.5 mM
13C-15N_GABPalpha_PNT: GABPalpha Pointed Domain, [U-99% 13C; U-99% 15N], 1.5 mM
10_13C-15N_GABPalpha_PNT: GABPalpha Pointed Domain, [N-10% 13C; U-99% 15N], 1.5 mM
sample_conditions: pH: 7.2; temperature: 303 K; ionic strength: 0.02 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | not available |
| 1H-13C HSQC | not available | not available | not available |
| HNCACB | not available | not available | not available |
| HBCBCACONH | not available | not available | not available |
| HNCO | not available | not available | not available |
| H(CCO)NH-TOCSY | not available | not available | not available |
| C(CO)NH-TOCSY | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
Software:
FELIX v95.0 - processing spectra, assignment
NMR spectrometers:
- Varian UNITY 500 MHz
Related Database Links:
| BMRB | 6287 |
| PDB | |
| DBJ | BAA02575 BAD96884 BAE24181 BAE26442 BAE26687 |
| GB | AAA53030 AAA65706 AAH13562 AAH35031 AAH52448 |
| REF | NP_001068905 NP_001102311 NP_001184226 NP_001253514 NP_002031 |
| SP | Q00422 Q06546 |
| TPG | DAA33658 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts