BMRB Entry 5470
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5470
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Title: Inhibitory Specificity Change of Ovomucoid Third Domain of Silver Pheasant upon Introduction of an Engineered Cys14-Cys39 Bond
Deposition date: 2002-07-22 Original release date: 2003-03-06
Authors: Hemmi, Hikaru; Kumazaki, Takashi; Yamazaki, Toshimasa; Kojima, Shuichi; Yoshida, Takuya; Kyogoku, Yoshimasa; Katsu, Masataka; Shinohara, Fumikazu; Yokosawa, Hideyoshi; Miura, Kin-ichiro; Kobayashi, Yuji
Citation: Hemmi, Hikaru; Kumazaki, Takashi; Yamazaki, Toshimasa; Kojima, Shuichi; Yoshida, Takuya; Kyogoku, Yoshimasa; Katsu, Masataka; Shinohara, Fumikazu; Yokosawa, Hideyoshi; Miura, Kin-ichiro; Kobayashi, Yuji. "Inhibitory Specificity Change of Ovomucoid Third Domain of Silver Pheasant upon Introduction of an Engineered Cys14-Cys39 Bond" Biochemistry 42, 2524-2534 (2003).
Assembly members:
ovomucoid third domain from silver pheasant, polymer, 54 residues, Formula weight is not available
Natural source: Common Name: silver pheasant Taxonomy ID: 9046 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Lophura nycthemera
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
ovomucoid third domain from silver pheasant: AVSVDCSEYPKCACTMEYRP
LCGSDNKTYGNKCNFCCAVV
ESNGTLTLSHFGKC
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 341 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | p14c/n39c monomer | 1 |
Entities:
Entity 1, p14c/n39c monomer 54 residues - Formula weight is not available
| 1 | ALA | VAL | SER | VAL | ASP | CYS | SER | GLU | TYR | PRO | ||||
| 2 | LYS | CYS | ALA | CYS | THR | MET | GLU | TYR | ARG | PRO | ||||
| 3 | LEU | CYS | GLY | SER | ASP | ASN | LYS | THR | TYR | GLY | ||||
| 4 | ASN | LYS | CYS | ASN | PHE | CYS | CYS | ALA | VAL | VAL | ||||
| 5 | GLU | SER | ASN | GLY | THR | LEU | THR | LEU | SER | HIS | ||||
| 6 | PHE | GLY | LYS | CYS |
Samples:
sample_1: ovomucoid third domain from silver pheasant 4.0 mM
Ex-cond_1: pH: 4.0; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-1H NOESY | not available | not available | not available |
| 1H-1H TOCSY | not available | not available | not available |
| 1H-1H DQF-COSY | not available | not available | not available |
Software:
NMRPipe - processing
PIPP - assignment, peak picking
NMR spectrometers:
- Bruker AMX 500 MHz
- Bruker DRX 600 MHz
Related Database Links:
| PDB |