BMRB Entry 5481
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5481
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Title: Backbone and side chain 1H, 13C, and 15N chemical shift assignments for S. cerevisae Hub1
Deposition date: 2002-07-25 Original release date: 2003-07-30
Authors: Ramelot, Theresa; Kennedy, Michael
Citation: Ramelot, Theresa; Cort, R.; Yee, A.; Semesi, A.; Edwards, A.; Arrowsmith, C.; Kennedy, Michael. "Solution structure of the yeast ubiquitin-like modifier protein Hub1" J. Struct. Funct. Genomics 4, 25-30 (2003).
Assembly members:
Hub1, polymer, 93 residues, 8272 Da.
Natural source: Common Name: Yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Hub1: MGSSHHHHHHSSGLVPRGSH
MIEVVVNDRLGKKVRVKCLA
EDSVGDFKKVLSLQIGTQPN
KIVLQKGGSVLKDHISLEDY
EVHDQTNLELYYL
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 537 |
| 13C chemical shifts | 321 |
| 15N chemical shifts | 75 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | hub1 | 1 |
Entities:
Entity 1, hub1 93 residues - 8272 Da.
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | SER | GLY | LEU | VAL | PRO | ARG | GLY | SER | HIS | ||||
| 3 | MET | ILE | GLU | VAL | VAL | VAL | ASN | ASP | ARG | LEU | ||||
| 4 | GLY | LYS | LYS | VAL | ARG | VAL | LYS | CYS | LEU | ALA | ||||
| 5 | GLU | ASP | SER | VAL | GLY | ASP | PHE | LYS | LYS | VAL | ||||
| 6 | LEU | SER | LEU | GLN | ILE | GLY | THR | GLN | PRO | ASN | ||||
| 7 | LYS | ILE | VAL | LEU | GLN | LYS | GLY | GLY | SER | VAL | ||||
| 8 | LEU | LYS | ASP | HIS | ILE | SER | LEU | GLU | ASP | TYR | ||||
| 9 | GLU | VAL | HIS | ASP | GLN | THR | ASN | LEU | GLU | LEU | ||||
| 10 | TYR | TYR | LEU |
Samples:
sample_1: Hub1, [U-15N; U-13C], 1 – 3 mM; acetate buffer 10 mM; NaCl 300 mM
sample_2: Hub1, [U-15N], 1 – 3 mM; acetate buffer 10 mM; NaCl 300 mM
sample_3: Hub1, [U-10% 13C; U-15N], 1 – 3 mM; acetate buffer 10 mM; NaCl 300 mM
cond_1: pH: 5.0; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 4D CC-NOESY and 1H-15N HSQC spectra were recorded after exchange into D2O. | not available | not available | cond_1 |
| 1H-13C HSQC was recorded on U-15N, 10% 13C labeled sample | not available | not available | cond_1 |
Software:
Felix v98 - processing data
XPLOR v3.84 - structure calculations
Sparky v3.98 - chemical shift and noesy assignments
NMR spectrometers:
- Varian Inova 600 MHz
- Varian Inova 750 MHz
- Varian Inova 800 MHz
Related Database Links:
| PDB | |
| DBJ | GAA26125 |
| EMBL | CAY82226 |
| GB | AAS56885 AHY77119 EDN62838 EDV12235 EEU08318 |
| PIR | S78735 |
| REF | NP_014430 |
| SP | Q6Q546 |
| TPG | DAA10573 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts