BMRB Entry 5496
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5496
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Title: NMR structure of ubiquitin-like domain in Parkin: Gene product of familial Parkinson's disease
Deposition date: 2002-08-07 Original release date: 2003-01-14
Authors: Tashiro, Mitsuru; Okubo, Seiji; Shimotakahara, Sakurako; Hatanaka, Hideki; Yasuda, Hideyo; Kainosho, Masatsune; Shigeyuki, Yokoyama; Shindo, Heisaburo
Citation: Tashiro, Mitsuru; Okubo, Seiji; Shimotakahara, Sakurako; Hatanaka, Hideki; Yasuda, Hideyo; Kainosho, Masatsune; Shigeyuki, Yokoyama; Shindo, Heisaburo. "Letter to the Editor: NMR structure of ubiquitin-like domain in PARKIN: Gene product of familial Parkinson's disease" J. Biomol. NMR 25, 153-156 (2003).
Assembly members:
ubiquitin-like domain in murine PARKIN, polymer, 78 residues, Formula weight is not available
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
ubiquitin-like domain in murine PARKIN: MGMIVFVRFNSSYGFPVEVD
SDTSILQLKEVVAKRQGVPA
DQLRVIFAGKELPNHLTVQN
CDLEQQSIVHIVQRPRRR
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 556 |
| 13C chemical shifts | 333 |
| 15N chemical shifts | 78 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Uld subunit 1 | 1 |
Entities:
Entity 1, Uld subunit 1 78 residues - Formula weight is not available
| 1 | MET | GLY | MET | ILE | VAL | PHE | VAL | ARG | PHE | ASN | ||||
| 2 | SER | SER | TYR | GLY | PHE | PRO | VAL | GLU | VAL | ASP | ||||
| 3 | SER | ASP | THR | SER | ILE | LEU | GLN | LEU | LYS | GLU | ||||
| 4 | VAL | VAL | ALA | LYS | ARG | GLN | GLY | VAL | PRO | ALA | ||||
| 5 | ASP | GLN | LEU | ARG | VAL | ILE | PHE | ALA | GLY | LYS | ||||
| 6 | GLU | LEU | PRO | ASN | HIS | LEU | THR | VAL | GLN | ASN | ||||
| 7 | CYS | ASP | LEU | GLU | GLN | GLN | SER | ILE | VAL | HIS | ||||
| 8 | ILE | VAL | GLN | ARG | PRO | ARG | ARG | ARG |
Samples:
sample_1: ubiquitin-like domain in murine PARKIN 1.0 mM
Uld-cond_1: pH: 5.0; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N NOESY | not available | not available | not available |
| 1H-13C NOESY | not available | not available | not available |
| HNCA | not available | not available | not available |
| HNCO | not available | not available | not available |
| CBCANH | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| C(CO)NH | not available | not available | not available |
| H(CCO)NH | not available | not available | not available |
Software:
NMRview -
NMRDraw -
NMR spectrometers:
- Bruker DMX 500 MHz
- Bruker DRX 600 MHz
- Varian INOVA 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAA82404 |
| GB | AAG13890 AAG13891 AAG13892 AAI13205 ACL93281 |
| REF | NP_057903 XP_006523397 XP_006523398 XP_006523399 XP_006523401 |
| SP | Q9WVS6 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts