BMRB Entry 5565
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5565
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for porcine MSP
Deposition date: 2002-10-30 Original release date: 2004-09-22
Authors: Wang, Iren; Lou, Yuan-Chao; Wu, Kuen-Phon; Wu, Shih-Hsiung; Chang, Wen-Chang; Chen, Chinpan
Citation: Wang, Iren; Lou, Yuan-Chao; Wu, Kuen-Phon; Wu, Shih-Hsiung; Chang, Wen-Chang; Chen, Chinpan. "Disulfide pairings and structure of porcine beta-microseminoprotein" The BMRB entry is the only known published source for the data..
Assembly members:
beta-microseminoprotein, polymer, 91 residues, Formula weight is not available
Natural source: Common Name: Pig Taxonomy ID: 9823 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Sus scrofa
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
beta-microseminoprotein: QCYFIPNQSLKPNECQDLKG
VSHPLNSVWKTKDCEECTCG
QDAISCCNTAAIPTGYDTNK
CQKILNKKTCTYTVVEKKDP
GKTCDVTGWVL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 394 |
| 15N chemical shifts | 97 |
| 1H chemical shifts | 603 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | porcine MSP | 1 |
Entities:
Entity 1, porcine MSP 91 residues - Formula weight is not available
| 1 | GLN | CYS | TYR | PHE | ILE | PRO | ASN | GLN | SER | LEU | ||||
| 2 | LYS | PRO | ASN | GLU | CYS | GLN | ASP | LEU | LYS | GLY | ||||
| 3 | VAL | SER | HIS | PRO | LEU | ASN | SER | VAL | TRP | LYS | ||||
| 4 | THR | LYS | ASP | CYS | GLU | GLU | CYS | THR | CYS | GLY | ||||
| 5 | GLN | ASP | ALA | ILE | SER | CYS | CYS | ASN | THR | ALA | ||||
| 6 | ALA | ILE | PRO | THR | GLY | TYR | ASP | THR | ASN | LYS | ||||
| 7 | CYS | GLN | LYS | ILE | LEU | ASN | LYS | LYS | THR | CYS | ||||
| 8 | THR | TYR | THR | VAL | VAL | GLU | LYS | LYS | ASP | PRO | ||||
| 9 | GLY | LYS | THR | CYS | ASP | VAL | THR | GLY | TRP | VAL | ||||
| 10 | LEU |
Samples:
Sample_1: beta-microseminoprotein, [U-13C; U-15N], 1.5 mM
Ex-cond_1: pH: 5.0; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | not available |
| 1H-15N NOESY | not available | not available | not available |
| 1H-15N TOCSY | not available | not available | not available |
| 1H-13C NOESY | not available | not available | not available |
| HN(CO)CA | not available | not available | not available |
| HN(CO)CACB | not available | not available | not available |
| HNCO | not available | not available | not available |
Software:
XWINNMR v2.5 - data processing
NMR spectrometers:
- Bruker AVANCE 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts