BMRB Entry 5578
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5578
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Title: Two Homologous Rat Cellular Retinol-binding Proteins Differ in Local Conformational Flexibility PubMed: 12850148
Deposition date: 2002-11-06 Original release date: 2003-07-25
Authors: Lu, Jianyun; Cistola, David; Li, Ellen
Citation: Lu, Jianyun; Cistola, David; Li, Ellen. "Two Homologous Rat Cellular Retinol-binding Proteins Differ in Local Conformational Flexibility" J. Mol. Biol. 330, 799-812 (2003).
Assembly members:
rat cellular retinol-binding protein I, polymer, 134 residues, Formula weight is not available
RETINOL, non-polymer, Formula weight is not available
Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
rat cellular retinol-binding protein I: PVDFNGYWKMLSNENFEEYL
RALDVNVALRKIANLLKPDK
EIVQDGDHMIIRTLSTFRNY
IMDFQVGKEFEEDLTGIDDR
KCMTTVSWDGDKLQCVQKGE
KEGRGWTQWIEGDELHLEMR
AEGVTCKQVFKKVH
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 969 |
| 13C chemical shifts | 550 |
| 15N chemical shifts | 148 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | holo-CRBP I | 1 |
| 2 | RETINOL | 2 |
Entities:
Entity 1, holo-CRBP I 134 residues - Formula weight is not available
| 1 | PRO | VAL | ASP | PHE | ASN | GLY | TYR | TRP | LYS | MET | ||||
| 2 | LEU | SER | ASN | GLU | ASN | PHE | GLU | GLU | TYR | LEU | ||||
| 3 | ARG | ALA | LEU | ASP | VAL | ASN | VAL | ALA | LEU | ARG | ||||
| 4 | LYS | ILE | ALA | ASN | LEU | LEU | LYS | PRO | ASP | LYS | ||||
| 5 | GLU | ILE | VAL | GLN | ASP | GLY | ASP | HIS | MET | ILE | ||||
| 6 | ILE | ARG | THR | LEU | SER | THR | PHE | ARG | ASN | TYR | ||||
| 7 | ILE | MET | ASP | PHE | GLN | VAL | GLY | LYS | GLU | PHE | ||||
| 8 | GLU | GLU | ASP | LEU | THR | GLY | ILE | ASP | ASP | ARG | ||||
| 9 | LYS | CYS | MET | THR | THR | VAL | SER | TRP | ASP | GLY | ||||
| 10 | ASP | LYS | LEU | GLN | CYS | VAL | GLN | LYS | GLY | GLU | ||||
| 11 | LYS | GLU | GLY | ARG | GLY | TRP | THR | GLN | TRP | ILE | ||||
| 12 | GLU | GLY | ASP | GLU | LEU | HIS | LEU | GLU | MET | ARG | ||||
| 13 | ALA | GLU | GLY | VAL | THR | CYS | LYS | GLN | VAL | PHE | ||||
| 14 | LYS | LYS | VAL | HIS |
Entity 2, RETINOL 1 residues - C20 H30 O1 - Formula weight is not available
| 1 | RTL |
Samples:
sample_1: rat cellular retinol-binding protein I, [U-99% 15N; U-13C], 1.0 mM
Sample_2: rat cellular retinol-binding protein I, [U-80% 2H; U-99% 15N], 1.0 mM
Ex-cond_1: pH: 7.4; temperature: 298 K
Ex-cond_2: pH: 6.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCO | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCACONNH | not available | not available | not available |
| CBCACOCAHA | not available | not available | not available |
| 1H-15N NOESY | not available | not available | not available |
| 1H-15N TOCSY | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
| 1H-13C NOESY | not available | not available | not available |
Software:
FELIX v2000 - automatic peak picking
NMR spectrometers:
- Varian INOVA 500 MHz
- Varian INOVA 600 MHz
- Varian UNITYPLUS 500 MHz
- Varian UNITY 600 MHz
Related Database Links:
| BMRB | 5048 5319 5330 5331 5579 |
| PDB | |
| DBJ | BAB27753 |
| EMBL | CAA42919 |
| GB | AAA35714 AAA40962 AAA42021 AAH18254 AAH91751 |
| REF | NP_035384 NP_036865 XP_005080789 XP_005366741 XP_006083819 |
| SP | P02696 Q00915 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
or all simulated shifts