BMRB Entry 5585
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5585
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Title: Isolation, structure and activity of GID, a novel alpha4/7-conotoxin with an extended N-terminal sequence PubMed: 12419800
Deposition date: 2002-11-12 Original release date: 2008-07-17
Authors: Nicke, A.; Loughnan, M.; Millard, E.; Alewood, P.; Adams, D.; Daly, N.; Craik, D.; Lewis, R.
Citation: Nicke, A.; Loughnan, M.; Millard, E.; Alewood, P.; Adams, D.; Daly, N.; Craik, D.; Lewis, R.. "Isolation, Structure, and Activity of GID, a Novel Alpha 4/7-conotoxin with an Extended N-terminal Sequence" J. Biol. Chem. 278, 3137-3144 (2003).
Assembly members:
alpha-conotoxin GID, polymer, 19 residues, Formula weight is not available
Natural source: Common Name: geography cone Taxonomy ID: 6491 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Conus Geographus
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
alpha-conotoxin GID: IRDXCCSNPACRVNNXHVC
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 90 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | alpha-conotoxin GID | 1 |
Entities:
Entity 1, alpha-conotoxin GID 19 residues - Formula weight is not available
| 1 | ILE | ARG | ASP | CGU | CYS | CYS | SER | ASN | PRO | ALA | ||||
| 2 | CYS | ARG | VAL | ASN | ASN | HYP | HIS | VAL | CYS |
Samples:
sample_1: alpha-conotoxin GID 2.5 mM; H2O 90%; D2O 10%
sample_cond_1: ionic strength: 0 M; pH: 2.8; pressure: 1 atm; temperature: 287 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D TOCSY | sample_1 | not available | sample_cond_1 |
| 2D NOESY | sample_1 | not available | sample_cond_1 |
| DQF-COSY | sample_1 | not available | sample_cond_1 |
| E-COSY | sample_1 | not available | sample_cond_1 |
Software:
XWINNMR v2.6 - collection
XEASY v1.3.7 - data analysis
DYANA v1.5 - structure solution
CNS v1.0 - refinement
NMR spectrometers:
- Bruker DMX 750 MHz
- Bruker ARX 500 MHz
Related Database Links:
| PDB |