BMRB Entry 5620
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5620
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Title: Assignment for ribosomal protein S28E
Deposition date: 2002-12-10 Original release date: 2002-12-27
Authors: Wu, Bin; Yee, Adelinda; Arrowsmith, Cheryl
Citation: Wu, Bin; Yee, Adelinda; Arrowsmith, Cheryl. "Solution structure of ribosomal protein S28E from Methanobacterium Thermoautotrophicum " The BMRB entry is the only known published source for the data..
Assembly members:
ribosomal protein S28E, polymer, 68 residues, 7666 Da.
Natural source: Common Name: Methanothermobacter thermoautotrophicus Taxonomy ID: 145262 Superkingdom: Archaea Kingdom: Euryarchaeota Genus/species: Methanobacterium thermoautotrophicum
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
ribosomal protein S28E: MDDATPAEVIEVLKRTGMTG
EVMQVKCRILDGRDKGRILT
RNVMGPIREGDILMLLDTIR
EAKEIRTP
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 483 |
| 13C chemical shifts | 281 |
| 15N chemical shifts | 58 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | mth0256 | 1 |
Entities:
Entity 1, mth0256 68 residues - 7666 Da.
| 1 | MET | ASP | ASP | ALA | THR | PRO | ALA | GLU | VAL | ILE | ||||
| 2 | GLU | VAL | LEU | LYS | ARG | THR | GLY | MET | THR | GLY | ||||
| 3 | GLU | VAL | MET | GLN | VAL | LYS | CYS | ARG | ILE | LEU | ||||
| 4 | ASP | GLY | ARG | ASP | LYS | GLY | ARG | ILE | LEU | THR | ||||
| 5 | ARG | ASN | VAL | MET | GLY | PRO | ILE | ARG | GLU | GLY | ||||
| 6 | ASP | ILE | LEU | MET | LEU | LEU | ASP | THR | ILE | ARG | ||||
| 7 | GLU | ALA | LYS | GLU | ILE | ARG | THR | PRO |
Samples:
sample_1: ribosomal protein S28E, [U-13C; U-15N], 1 MM; NaCl 450 mM; Na2PO4 25 mM
cond_set1: pH: 6.5 na; temperature: 298 K; ionic strength: 0.45 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 13C-edited NOESY and 15N-edited NOESY were calibrated to obtain accurate | sample_1 | not available | cond_set1 |
| peakposition for structure calculation. | sample_1 | not available | cond_set1 |
Software:
XEASY - peak assignment
SPARKY v3.95 - peak assignment
NMR spectrometers:
- VARIAN INOVA 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAM69468 |
| GB | AAB84762 ADL58301 |
| REF | NP_275399 WP_010875895 WP_013295525 YP_003849614 |
| SP | O26356 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
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