BMRB Entry 5659
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5659
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Title: 1H, 13C and 15N Backbone Assignments for the Human Rhinovirus-14 3C Protease
Deposition date: 2003-01-13 Original release date: 2006-07-21
Authors: Bjorndahl, Trent; Monzavi, Hassan; Wishart, David
Citation: Bjorndahl, Trent; Monzavi, Hassan; Wishart, David. "Letter to the Editor: Backbone 1H, 15N and 13C assignments for the human rhinovirus 3C protease (serotype 14) " J. Biomol. NMR 26, 85-86 (2003).
Assembly members:
HRV14-3C, polymer, 182 residues, 19996.90 Da.
Natural source: Common Name: Human Rhinovirus-14 Taxonomy ID: 12131 Superkingdom: Virus Kingdom: Picornaviridae Genus/species: Rhinovirus not available
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
HRV14-3C: GPNTEFALSLLRKNIMTITT
SKGEFTGLGIHDRVCVIPTH
AQPGDDVLVNGQKIRVKDKY
KLVDPENINLELTVLTLDRN
EKFRDIRGFISEDLEGVDAT
LVVHSNNFTNTILEVGPVTM
AGLINLSSTPTNRMIRYDYA
TKTGQCGGVLCATGKIFGIH
VGGNGRQGFSAQLKKQYFVE
KQ
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 1163 |
| 13C chemical shifts | 683 |
| 15N chemical shifts | 159 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Human Rhinovirus-14 3C Protease | 1 |
Entities:
Entity 1, Human Rhinovirus-14 3C Protease 182 residues - 19996.90 Da.
| 1 | GLY | PRO | ASN | THR | GLU | PHE | ALA | LEU | SER | LEU | ||||
| 2 | LEU | ARG | LYS | ASN | ILE | MET | THR | ILE | THR | THR | ||||
| 3 | SER | LYS | GLY | GLU | PHE | THR | GLY | LEU | GLY | ILE | ||||
| 4 | HIS | ASP | ARG | VAL | CYS | VAL | ILE | PRO | THR | HIS | ||||
| 5 | ALA | GLN | PRO | GLY | ASP | ASP | VAL | LEU | VAL | ASN | ||||
| 6 | GLY | GLN | LYS | ILE | ARG | VAL | LYS | ASP | LYS | TYR | ||||
| 7 | LYS | LEU | VAL | ASP | PRO | GLU | ASN | ILE | ASN | LEU | ||||
| 8 | GLU | LEU | THR | VAL | LEU | THR | LEU | ASP | ARG | ASN | ||||
| 9 | GLU | LYS | PHE | ARG | ASP | ILE | ARG | GLY | PHE | ILE | ||||
| 10 | SER | GLU | ASP | LEU | GLU | GLY | VAL | ASP | ALA | THR | ||||
| 11 | LEU | VAL | VAL | HIS | SER | ASN | ASN | PHE | THR | ASN | ||||
| 12 | THR | ILE | LEU | GLU | VAL | GLY | PRO | VAL | THR | MET | ||||
| 13 | ALA | GLY | LEU | ILE | ASN | LEU | SER | SER | THR | PRO | ||||
| 14 | THR | ASN | ARG | MET | ILE | ARG | TYR | ASP | TYR | ALA | ||||
| 15 | THR | LYS | THR | GLY | GLN | CYS | GLY | GLY | VAL | LEU | ||||
| 16 | CYS | ALA | THR | GLY | LYS | ILE | PHE | GLY | ILE | HIS | ||||
| 17 | VAL | GLY | GLY | ASN | GLY | ARG | GLN | GLY | PHE | SER | ||||
| 18 | ALA | GLN | LEU | LYS | LYS | GLN | TYR | PHE | VAL | GLU | ||||
| 19 | LYS | GLN |
Samples:
sample_1: HRV14-3C, [U-15N], 1 mM; KH2PO4 20 mM; NaCl 0 mM
sample_2: HRV14-3C, [U-15N; U-13C], 1 mM; KH2PO4 20 mM; NaCl 0 mM
conditions_1: pH: 6.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCO | not available | not available | not available |
| HNCA | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCACONNH | not available | not available | not available |
| HNHA | not available | not available | not available |
| 15N-HSQC | not available | not available | not available |
Software:
NMRPIPE - specta processing
NMRVIEW - peak picking, spectral analysis
NMR spectrometers:
- Varian Unity 500 MHz
Related Database Links:
| PDB | |
| EMBL | CAA25565 |
| GB | AAA45756 AAA45757 AAA45758 |
| REF | NP_041009 NP_740524 |
| SP | P03303 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts