BMRB Entry 5673
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5673
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Title: NMR Solution Structure of the Glucagon Antagonist [desHis1, desPhe6, Glu9]Glucagon Amide in the Presence of Perdeuterated Dodecylphosphocholine Micelles PubMed: 12627948
Deposition date: 2003-01-23 Original release date: 2008-07-17
Authors: Ying, J.; Ahn, J.-M.; Jacobsen, N.; Brown, M.; Hruby, V.
Citation: Ying, J.; Ahn, J.-M.; Jacobsen, N.; Brown, M.; Hruby, V.. "NMR Solution Structure of the Glucagon Antagonist [desHis(1), desPhe(6), Glu(9)] Glucagon Amide in the Presence of Perdeuterated Dodecylphosphocholine Micelles" Biochemistry 42, 2825-2835 (2003).
Assembly members:
Glucagon analogue, polymer, 28 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapien
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
Glucagon analogue: SQGTTSEYSKYLDSRRAQDF
VQWLMNTX
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 144 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Glucagon analogue | 1 |
Entities:
Entity 1, Glucagon analogue 28 residues - Formula weight is not available
| 1 | SER | GLN | GLY | THR | THR | SER | GLU | TYR | SER | LYS | ||||
| 2 | TYR | LEU | ASP | SER | ARG | ARG | ALA | GLN | ASP | PHE | ||||
| 3 | VAL | GLN | TRP | LEU | MET | ASN | THR | NH2 |
Samples:
sample_1: Glucagon analogue 7 mM; sodium phosphate buffer 50 mM; dodecylphosphocholine-d38 283 mM; sodium azide 1 mM; H2O 90%; D2O 10%
sample_cond_1: ionic strength: 50 mM; pH: 6.0; pressure: 1 atm; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| DQF-COSY | sample_1 | not available | sample_cond_1 |
| 2D TOCSY | sample_1 | not available | sample_cond_1 |
| 2D NOESY | sample_1 | not available | sample_cond_1 |
Software:
UXNMR - collection
FELIX v2000 - data analysis, processing
DGII vwithin InsightII 2000 - structure solution
DISCOVER vwithin InsightII 2000 - refinement
NMR spectrometers:
- Bruker AMX 500 MHz
Related Database Links:
| PDB |