BMRB Entry 5677
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5677
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Title: 1H, 13C, and 15N assignment of the Focal Adhesion Targeting Domain of Focal Adhesion Kinase PubMed: 14662767
Deposition date: 2003-01-24 Original release date: 2004-03-15
Authors: Prutzman, Kirk; Gao, Guanghua; Schaller, Michael; Campbell, Sharon
Citation: Gao, Guanghua; Prutzman, Kirk; King, M; Scheswohl, D; DeRose, E; London, R; Schaller, Michael; Campbell, Sharon. "NMR solution structure of the focal adhesion targeting domain of focal adhesion kinase in complex with a paxillin LD peptide: evidence for a two-site binding model." J. Biol. Chem. 279, 8441-8451 (2004).
Assembly members:
focal adhesion targeting domain, polymer, 146 residues, 15878 Da.
Natural source: Common Name: Chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
focal adhesion targeting domain: GSPGISGGGGGIRSNDKVYE
NVTGLVKAVIEMSSKIQPAP
PEEYVPMVKEVGLALRTLLA
TVDESLPVLPASTHREIEMA
QKLLNSDLAELINKMKLAQQ
YVMTSLQQEYKKQMLTAAHA
LAVDAKNLLDVIDQARLKMI
SQSRPH
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 803 |
| 13C chemical shifts | 560 |
| 15N chemical shifts | 143 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | FAT | 1 |
Entities:
Entity 1, FAT 146 residues - 15878 Da.
| 1 | GLY | SER | PRO | GLY | ILE | SER | GLY | GLY | GLY | GLY | ||||
| 2 | GLY | ILE | ARG | SER | ASN | ASP | LYS | VAL | TYR | GLU | ||||
| 3 | ASN | VAL | THR | GLY | LEU | VAL | LYS | ALA | VAL | ILE | ||||
| 4 | GLU | MET | SER | SER | LYS | ILE | GLN | PRO | ALA | PRO | ||||
| 5 | PRO | GLU | GLU | TYR | VAL | PRO | MET | VAL | LYS | GLU | ||||
| 6 | VAL | GLY | LEU | ALA | LEU | ARG | THR | LEU | LEU | ALA | ||||
| 7 | THR | VAL | ASP | GLU | SER | LEU | PRO | VAL | LEU | PRO | ||||
| 8 | ALA | SER | THR | HIS | ARG | GLU | ILE | GLU | MET | ALA | ||||
| 9 | GLN | LYS | LEU | LEU | ASN | SER | ASP | LEU | ALA | GLU | ||||
| 10 | LEU | ILE | ASN | LYS | MET | LYS | LEU | ALA | GLN | GLN | ||||
| 11 | TYR | VAL | MET | THR | SER | LEU | GLN | GLN | GLU | TYR | ||||
| 12 | LYS | LYS | GLN | MET | LEU | THR | ALA | ALA | HIS | ALA | ||||
| 13 | LEU | ALA | VAL | ASP | ALA | LYS | ASN | LEU | LEU | ASP | ||||
| 14 | VAL | ILE | ASP | GLN | ALA | ARG | LEU | LYS | MET | ILE | ||||
| 15 | SER | GLN | SER | ARG | PRO | HIS |
Samples:
Sample_1: focal adhesion targeting domain, [U-13C; U-15N], 0.85 mM
FAT-cond: pH: 6.0; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
NMRview v5.0.4 -
NMRPipe -
NMR spectrometers:
- Varian INOVA 800 MHz
- Varian INOVA 600 MHz
Related Database Links:
| BMRB | 5266 5924 |
| PDB | |
| GB | AAA48765 AAA48773 EMC80181 EOA99770 KFM11460 |
| REF | NP_990766 XP_002191290 XP_004174254 XP_005021656 XP_005021657 |
| SP | Q00944 |
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