BMRB Entry 5757
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5757
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Title: 13C, 15N solid state NMR chemical shift assignments for the microcrystallin Crh domain swapped dimer
Deposition date: 2003-03-27 Original release date: 2003-10-16
Authors: Bockmann, Anja; Lange, Adam; Galinier, Anne; Luca, Sorin; Giraud, Nicolas; Juy, Michel; Heise, Henrike; Montserret, Roland; Penin, Francois; Baldus, Marc
Citation: Bockmann, Anja; Lange, Adam; Galinier, Anne; Luca, Sorin; Giraud, Nicolas; Juy, Michel; Heise, Henrike; Montserret, Roland; Penin, Francois; Baldus, Marc. "Solid state NMR sequential resonance assignments and conformational analysis of the 2x10.4 kDa dimeric form of the Bacillus subtilis protein Crh" J. Biomol. NMR 27, 323-339 (2003).
Assembly members:
catabolite repression HPr, polymer, 93 residues, 10391 Da.
Natural source: Common Name: Bacillus subtilis Taxonomy ID: 1423 Superkingdom: Eubacteria Kingdom: not available Genus/species: Bacillus subtilis
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
catabolite repression HPr: MVQQKVEVRLKTGLQARPAA
LFVQEANRFTSDVFLEKDGK
KVNAKSIMGLMSLAVSTGTE
VTLIAQGEDEQEALEKLAAY
VQEEVLQHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 90 |
| 13C chemical shifts | 360 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Crh subunit 1 | 1 |
| 2 | Crh subunit 2 | 1 |
Entities:
Entity 1, Crh subunit 1 93 residues - 10391 Da.
| 1 | MET | VAL | GLN | GLN | LYS | VAL | GLU | VAL | ARG | LEU | ||||
| 2 | LYS | THR | GLY | LEU | GLN | ALA | ARG | PRO | ALA | ALA | ||||
| 3 | LEU | PHE | VAL | GLN | GLU | ALA | ASN | ARG | PHE | THR | ||||
| 4 | SER | ASP | VAL | PHE | LEU | GLU | LYS | ASP | GLY | LYS | ||||
| 5 | LYS | VAL | ASN | ALA | LYS | SER | ILE | MET | GLY | LEU | ||||
| 6 | MET | SER | LEU | ALA | VAL | SER | THR | GLY | THR | GLU | ||||
| 7 | VAL | THR | LEU | ILE | ALA | GLN | GLY | GLU | ASP | GLU | ||||
| 8 | GLN | GLU | ALA | LEU | GLU | LYS | LEU | ALA | ALA | TYR | ||||
| 9 | VAL | GLN | GLU | GLU | VAL | LEU | GLN | HIS | HIS | HIS | ||||
| 10 | HIS | HIS | HIS |
Samples:
sample_1: catabolite repression HPr, [U-98% 13C; U-98% 15N], 4 – 20 mg
Ex-cond_1: pH: 6.9; temperature: 278 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 13C-13C proton driven spin diffusion (PDSD) | sample_1 | not available | Ex-cond_1 |
| 13C-13C CO selective J-decoupled PDSD | sample_1 | not available | Ex-cond_1 |
| 13C-13C RFDR | sample_1 | not available | Ex-cond_1 |
| 13C-13C DQ SPC-5 | sample_1 | not available | Ex-cond_1 |
| 15N-13C NCACO | sample_1 | not available | Ex-cond_1 |
| 15N-13C NCOCACB | sample_1 | not available | Ex-cond_1 |
Software:
NMRPipe v2.0 - data processing
NMRVIEW v5.0 - assignments
NMR spectrometers:
- BRUKER DSX 500 MHz
- BRUKER DSX 600 MHz