BMRB Entry 5764
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5764
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Title: 1H, 13C and 15N Chemical Shift Assignments for the Nuclear Factor ALY RBD Domain PubMed: 12809490
Deposition date: 2003-04-07 Original release date: 2003-08-08
Authors: Perez-Alvarado, Gabriela; Martinez-Yamout, Maria; Allen, Melissa; Grosschedl, Rudolf; Dyson, H.; Wright, Peter
Citation: Perez-Alvarado, Gabriela; Martinez-Yamout, Maria; Allen, Melissa; Grosschedl, Rudolf; Dyson, H.; Wright, Peter. "Structure of the Nuclear Factor ALY: Insights into Post-transcriptional Regulatory and mRNA Nuclear Export Processes" Biochemistry 42, 7348-7357 (2003).
Assembly members:
ALY, polymer, 106 residues, Formula weight is not available
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia Escherichia coli
Entity Sequences (FASTA):
ALY: SRPKQLPDKWQHDLFDSGFG
GGAGVETGGKLLVSNLDFGV
SDADIQELFAEFGTLKKAAV
HYDRSGRSLGTADVHFERKA
DALKAMKQYNGVPLDGRPMN
IQLVTS
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 699 |
| 13C chemical shifts | 419 |
| 15N chemical shifts | 111 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ALY monomer | 1 |
Entities:
Entity 1, ALY monomer 106 residues - Formula weight is not available
| 1 | SER | ARG | PRO | LYS | GLN | LEU | PRO | ASP | LYS | TRP | ||||
| 2 | GLN | HIS | ASP | LEU | PHE | ASP | SER | GLY | PHE | GLY | ||||
| 3 | GLY | GLY | ALA | GLY | VAL | GLU | THR | GLY | GLY | LYS | ||||
| 4 | LEU | LEU | VAL | SER | ASN | LEU | ASP | PHE | GLY | VAL | ||||
| 5 | SER | ASP | ALA | ASP | ILE | GLN | GLU | LEU | PHE | ALA | ||||
| 6 | GLU | PHE | GLY | THR | LEU | LYS | LYS | ALA | ALA | VAL | ||||
| 7 | HIS | TYR | ASP | ARG | SER | GLY | ARG | SER | LEU | GLY | ||||
| 8 | THR | ALA | ASP | VAL | HIS | PHE | GLU | ARG | LYS | ALA | ||||
| 9 | ASP | ALA | LEU | LYS | ALA | MET | LYS | GLN | TYR | ASN | ||||
| 10 | GLY | VAL | PRO | LEU | ASP | GLY | ARG | PRO | MET | ASN | ||||
| 11 | ILE | GLN | LEU | VAL | THR | SER |
Samples:
sample_1: ALY, [U-15N], 0.5 mM; Tris-HCl-d8 20 mM; NaCl 100 mM; H2O 90%; D2O 10%
sample_2: ALY, [U-15N; U-13C], 0.5 mM; Tris-HCl-d8 20 mM; NaCl 100 mM; H2O 90%; D2O 10%
sample_cond_1: pH: 6.5; temperature: 296 K; ionic strength: 100 mM; pressure: 1 atm
sample_cond_2: pH: 6.5; temperature: 289 K; ionic strength: 100 mM; pressure: 1 atm
sample_cond_3: pH: 5.9; temperature: 296 K; ionic strength: 100 mM; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated_NOESY | not available | not available | not available |
| 3D 13C-HMQC-NOESY | not available | not available | not available |
| 3D 15N/13C-NOESY-HSQC | not available | not available | not available |
| HNCA | not available | not available | not available |
| HNCO | not available | not available | not available |
| HN(CA)CB | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| C(CO)NH-TOCSY | not available | not available | not available |
| HC(CO)NH-TOCSY | not available | not available | not available |
| HNHA | not available | not available | not available |
| HCCH-COSY | not available | not available | not available |
| CCH-COSY | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
| HNHB | not available | not available | not available |
| 2D TOCSY | not available | not available | not available |
| 3D 13C-separated_NOESY | not available | not available | not available |
Software:
XWINNMR v2.1 - collection
NMRPipe v1.8, 2.1 - processing
NMRDraw v1.8, 2.1 - processing
NMRVIEW v3 - data analysis
DYANA v1.5 - structure solution
AMBER v6, 7 - refinement
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker DRX 800 MHz
- Bruker AMX 500 MHz
Related Database Links:
| PDB | |
| EMBL | CAB76383 CAJ81934 |
| GB | AAC53117 AAD09608 AAH52302 AAH92013 AAI02385 |
| REF | NP_001017027 NP_001030494 NP_001089314 NP_001103072 NP_001232343 |
| SP | B5FXN8 O08583 Q28FB9 Q3T0I4 Q58EA2 |
| TPG | DAA18257 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts