BMRB Entry 5781
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5781
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Title: The Solution Structure of a DNA.RNA Duplex Containing 5-Propynyl U and C Comparison with 5-Me Modifications PubMed: 12736318
Deposition date: 2003-04-20 Original release date: 2003-05-19
Authors: Gyi, J.; Gao, D.; Conn, G.; Trent, J.; Brown, T.; Lane, A.
Citation: Gyi, J.; Gao, D.; Conn, G.; Trent, J.; Brown, T.; Lane, A.. "The Solution Structure of a DNA.RNA Duplex Containing 5-Propynyl U and C; Comparison with 5-Me Modifications" Nucleic Acids Res. 31, 2683-2693 (2003).
Assembly members:
DNA.RNA_hybrid, polymer, 10 residues, 6000 Da.
DNA.RNA_hybrid, polymer, 10 residues, 6000 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
DNA.RNA_hybrid: GCXXCXCXXC
DNA.RNA_hybrid: GAAGAGAAGC
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 175 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Propynyl_DNA.RNA_hybrid chain A | 1 |
| 2 | Propynyl_DNA.RNA_hybrid chain B | 2 |
Entities:
Entity 1, Propynyl_DNA.RNA_hybrid chain A 10 residues - 6000 Da.
| 1 | DG | DC | DU | DU | DC | DU | DC | DU | DU | DC |
Entity 2, Propynyl_DNA.RNA_hybrid chain B 10 residues - 6000 Da.
| 1 | G | A | A | G | A | G | A | A | G | C |
Samples:
sample_1: DNA.RNA_hybrid 1 mM; DNA.RNA_hybrid 1 mM; D2O 100%; KCL 100 mM
sample_2: DNA.RNA_hybrid 1 mM; DNA.RNA_hybrid 1 mM; H2O 90%; D2O 10%; KCL 100 mM
sample_cond_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 ATM; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NOESY | not available | not available | sample_cond_1 |
| COSY | not available | not available | sample_cond_1 |
| 2Q COSY(INADEQUATE) | not available | not available | sample_cond_1 |
Software:
AMBER v7.0 - refinement
FELIX v95.0 - processing
NUCFIT v0.0012 - data analysis
PFIT v1.1 - data analysis
NMR spectrometers:
- VARIAN UNITY PLUS 600 MHz
- VARIAN UNITY 500 MHz