BMRB Entry 5851
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5851
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Solution Stucture of the Third Zinc Finger from BKLF PubMed: 12736264
Deposition date: 2003-06-26 Original release date: 2003-09-12
Authors: Simpson, R.; Cram, E.; Czolij, R.; Matthews, J.; Crossley, M.; Mackay, J.
Citation: Simpson, R.; Cram, E.; Czolij, R.; Matthews, J.; Crossley, M.; Mackay, J.. "CCHX Zinc Finger Derivatives Retain the Ability to Bind Zn(II) and Mediate Protein-DNA Interactions" J. Biol. Chem. 278, 28011-28018 (2003).
Assembly members:
Kruppel-like factor 3, polymer, 37 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Kruppel-like factor 3: GSTRGSTGIKPFQCPDCDRS
FSRSDHLALHRKRHMLV
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 15N chemical shifts | 33 |
| 1H chemical shifts | 213 |
| coupling constants | 28 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Kruppel-like factor 3 | 1 |
| 2 | ZINC ION | 2 |
Entities:
Entity 1, Kruppel-like factor 3 37 residues - Formula weight is not available
| 1 | GLY | SER | THR | ARG | GLY | SER | THR | GLY | ILE | LYS | ||||
| 2 | PRO | PHE | GLN | CYS | PRO | ASP | CYS | ASP | ARG | SER | ||||
| 3 | PHE | SER | ARG | SER | ASP | HIS | LEU | ALA | LEU | HIS | ||||
| 4 | ARG | LYS | ARG | HIS | MET | LEU | VAL |
Entity 2, ZINC ION - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: Kruppel-like factor 3 3 mM; TCEP 4.5 mM; ZnSO4 4.5 mM; H2O 90%; D2O 10%
sample_2: Kruppel-like factor 3, [U-15N], 1 mM; TCEP 1.5 mM; ZnSO4 1.5 mM; H2O 90%; D2O 10%
sample_cond_1: pH: 5.5; pressure: 1 atm; temperature: 280 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | not available | not available | sample_cond_1 |
| 2D TOCSY | not available | not available | sample_cond_1 |
| DQF-COSY | not available | not available | sample_cond_1 |
| HNHA | not available | not available | sample_cond_1 |
Software:
xwinnmr v2.5 - processing
DYANA v1.5 -
XEASY v1.3.13 - data analysis
ARIA v1.1.2 - refinement, structure solution
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| PDB |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts