BMRB Entry 5868
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5868
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Title: Solution structure of XPC binding domain of hHR23B PubMed: 15885096
Deposition date: 2003-07-14 Original release date: 2005-05-19
Authors: Kim, B.; Ryu, K.-S.; Kim, H.; Choi, B.-S.
Citation: Kim, B.; Ryu, K.-S.; Kim, H.; Cho, S.; Choi, B.-S.. "Solution structure and backbone dynamics of the XPC-binding domain of the human DNA repair protein hHR23B." FEBS J. 272, 2467-2476 (2005).
Assembly members:
RAD23 homolog B, polymer, 72 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
RAD23 homolog B: GSHMPLEFLRNQPQFQQMRQ
IIQQNPSLLPALLQQIGREN
PQLLQQISQHQEHFIQMLNE
PVQEAGGQGGGG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 269 |
| 15N chemical shifts | 82 |
| 1H chemical shifts | 461 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RAD23 homolog B | 1 |
Entities:
Entity 1, RAD23 homolog B 72 residues - Formula weight is not available
| 1 | GLY | SER | HIS | MET | PRO | LEU | GLU | PHE | LEU | ARG | ||||
| 2 | ASN | GLN | PRO | GLN | PHE | GLN | GLN | MET | ARG | GLN | ||||
| 3 | ILE | ILE | GLN | GLN | ASN | PRO | SER | LEU | LEU | PRO | ||||
| 4 | ALA | LEU | LEU | GLN | GLN | ILE | GLY | ARG | GLU | ASN | ||||
| 5 | PRO | GLN | LEU | LEU | GLN | GLN | ILE | SER | GLN | HIS | ||||
| 6 | GLN | GLU | HIS | PHE | ILE | GLN | MET | LEU | ASN | GLU | ||||
| 7 | PRO | VAL | GLN | GLU | ALA | GLY | GLY | GLN | GLY | GLY | ||||
| 8 | GLY | GLY |
Samples:
sample_1: RAD23 homolog B, [U-15N; U-13C], 1 mM; phosphate buffer NA 40 mM; CHAPS NA 10 mM; H2O 90%; D2O 10%
sample_cond_1: ionic strength: 210 mM; pH: 7.0; pressure: 1 atm; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | not available | not available | not available |
| 3D 13C-separated NOESY | not available | not available | not available |
| HNHA | not available | not available | not available |
Software:
VNMR v6.3c - collection
NMRPipe - processing
CYANA v1.6 - structure solution
AMBER v7 - refinement
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAA04652 BAD97199 BAE89144 BAE89405 BAG54170 |
| EMBL | CAA63146 |
| GB | AAH20973 AAH27747 AAH70960 AAH90351 AAH98674 |
| PRF | 2206377B |
| REF | NP_001020446 NP_001039775 NP_001231638 NP_001231642 NP_001231653 |
| SP | P54727 Q29RK4 Q4KMA2 |
| TPG | DAA26518 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts