BMRB Entry 5905
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5905
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Title: Backbone 15N and 1HN Chemical Shift Assignments for Sac7d
Deposition date: 2003-08-18 Original release date: 2007-07-11
Authors: Clark, Andrew
Citation: Clark, Andrew; McCrary, Bradford; Edmondson, Stephen; Shriver, John. "Thermodynamics of Hydrophobic Core Packing in the Thermophile proteins Sac7d and Sso7d" J. Mol. Biol. ., .-..
Assembly members:
Sac7d, polymer, 66 residues, 7609 Da.
Natural source: Common Name: Sulfolobus acidocaldarius Brock et al Taxonomy ID: 2285 Superkingdom: Archaea Kingdom: not available Genus/species: Sulfolobus acidocaldarius
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Sac7d: MVKVKFKYKGEEKEVDTSKI
KKVWRVGKMVSFTYDDNGKT
GRGAVSEKDAPKELLDMLAR
AEREKK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 67 |
| 13C chemical shifts | 13 |
| 15N chemical shifts | 66 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Sac7d monomer | 1 |
Entities:
Entity 1, Sac7d monomer 66 residues - 7609 Da.
| 1 | MET | VAL | LYS | VAL | LYS | PHE | LYS | TYR | LYS | GLY | ||||
| 2 | GLU | GLU | LYS | GLU | VAL | ASP | THR | SER | LYS | ILE | ||||
| 3 | LYS | LYS | VAL | TRP | ARG | VAL | GLY | LYS | MET | VAL | ||||
| 4 | SER | PHE | THR | TYR | ASP | ASP | ASN | GLY | LYS | THR | ||||
| 5 | GLY | ARG | GLY | ALA | VAL | SER | GLU | LYS | ASP | ALA | ||||
| 6 | PRO | LYS | GLU | LEU | LEU | ASP | MET | LEU | ALA | ARG | ||||
| 7 | ALA | GLU | ARG | GLU | LYS | LYS |
Samples:
sample: Sac7d, [U-98% 15N], 1.2 mM; KCl 0.3 M; H2O 90%; D2O 10%; DSS 100 uM
conditions: pH: 4.07 na; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H,15N HSQC | sample | not available | conditions |
| 3D HSQC-TOCSY | sample | not available | conditions |
| 3D HSQC-NOESY | sample | not available | conditions |
Software:
NMRPipe v5 - data processing, transformation, referencing
NMRView - sequential assignments
NMR spectrometers:
- Varian VXR-S 500 MHz
- Varian INOVA 500 MHz
- Varian INOVA 800 MHz
Related Database Links:
| BMRB | 5908 |
| PDB | |
| GB | AAA80315 AAY79492 AGE70041 AGE72316 AHC50588 |
| REF | WP_011276993 YP_007433189 YP_007435464 YP_254785 |
| SP | P13123 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts