BMRB Entry 5932
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5932
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Title: YNMG tetraloop formation by a dyskeratosis congenita mutation in human telomerase RNA PubMed: 14624001
Deposition date: 2003-09-04 Original release date: 2003-12-19
Authors: Theimer, C.; Finger, L.; Feigon, J.
Citation: Theimer, C.; Finger, L.; Feigon, J.. "YNMG tetraloop formation by a dyskeratosis congenita mutation in human telomerase RNA" RNA 9, 1446-1455 (2003).
Assembly members:
human telomerase RNA P2b loop, polymer, 15 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
human telomerase RNA P2b loop: GGCUCUCAGUGAGCC
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 100 |
| 15N chemical shifts | 31 |
| 1H chemical shifts | 136 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | dcloop | 1 |
Entities:
Entity 1, dcloop 15 residues - Formula weight is not available
| 1 | G | G | C | U | C | U | C | A | G | U | ||||
| 2 | G | A | G | C | C |
Samples:
sample_1: human telomerase RNA P2b loop 1 mM; sodium phosphate buffer 10 mM; KCl 200 mM; EDTA 50 uM; sodium azide 0.2%; H2O 95%; D2O 5%
sample_2: human telomerase RNA P2b loop, [U-13C; U-15N], 1 mM; sodium phosphate buffer 10 mM; KCl 200 mM; EDTA 50 uM; sodium azide 0.2%; H2O 95%; D2O 5%
sample_3: human telomerase RNA P2b loop 1 mM; sodium phosphate buffer 10 mM; KCl 200 mM; EDTA 50 uM; sodium azide 0.2%; D2O 100%
sample_4: human telomerase RNA P2b loop, [U-13C; U-15N], 1 mM; sodium phosphate buffer 10 mM; KCl 200 mM; EDTA 50 uM; sodium azide 0.2%; D2O 100%
sample_cond_1: ionic strength: 200 mM; pH: 6.3; pressure: 1 atm; temperature: 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY (1,1echo and watergate) | not available | not available | not available |
| 2D 15N-HMQC | not available | not available | not available |
| 2D 15N-CPMG-NOESY | not available | not available | not available |
| 2D JNN-HNN-COSY | not available | not available | not available |
| 2D NOESY | not available | not available | not available |
| 2D TOCSY | not available | not available | not available |
| 31P-spin echo difference CT HSQC | not available | not available | not available |
| 3D 13C-NOESY HMQC | not available | not available | not available |
| 2D 13C-HMQC | not available | not available | not available |
| 2D HCCH COSY | not available | not available | not available |
| 3D HCCH TOCSY | not available | not available | not available |
| CT-CE-HSQC | not available | not available | not available |
Software:
xwinnmr v2.6 - collection, processing
AURELIA v3.108 - data analysis
X-PLOR v3.851 - refinement, structure solution
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker DRX 500 MHz