BMRB Entry 5939
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
BMRB Entry DOI: doi:10.13018/BMR5939
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Title: Backbone and sidechain assignment of murine Spred2 EVH1 domain PubMed: 15213456
Deposition date: 2003-09-09 Original release date: 2007-05-04
Authors: Fossi, Michele; Zimmermann, Juergen; Jarchau, Thomas; Lemak, Alexander; Walter, Ulrich; Wiegelt, Johan; Sundstrom, Michael; Arrowsmith, Cheryl; Edwards, Aled; Oschkinat, Hartmut; Ball, Linda
Citation: Zimmermann, Juergen; Jarchau, Thomas; Walter, Ulrich; Oschkinat, Hartmut; Ball, Linda. "Letter to the Editor: 1H, 13C and 15N resonance assignment of the human Spred2 EVH1 domain" J. Biomol. NMR 29, 435-436 (2004).
Assembly members:
murine Spred2 EVH1 domain, polymer, 126 residues, 14193 Da.
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
murine Spred2 EVH1 domain: GSMTEETHPDDDSYIVRVKA
VVMTRDDSSGGWFPQEGGGI
SRVGVCKVMHPEGNGRSGFL
IHGERQKDKLVVLECYVRKD
LVYTKANPTFHHWKVDNRKF
GLTFQSPADARAFDRGVRKA
IEDLIE
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 810 |
| 13C chemical shifts | 353 |
| 15N chemical shifts | 127 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Spred2 EVH1 domain | 1 |
Entities:
Entity 1, Spred2 EVH1 domain 126 residues - 14193 Da.
| 1 | GLY | SER | MET | THR | GLU | GLU | THR | HIS | PRO | ASP | ||||
| 2 | ASP | ASP | SER | TYR | ILE | VAL | ARG | VAL | LYS | ALA | ||||
| 3 | VAL | VAL | MET | THR | ARG | ASP | ASP | SER | SER | GLY | ||||
| 4 | GLY | TRP | PHE | PRO | GLN | GLU | GLY | GLY | GLY | ILE | ||||
| 5 | SER | ARG | VAL | GLY | VAL | CYS | LYS | VAL | MET | HIS | ||||
| 6 | PRO | GLU | GLY | ASN | GLY | ARG | SER | GLY | PHE | LEU | ||||
| 7 | ILE | HIS | GLY | GLU | ARG | GLN | LYS | ASP | LYS | LEU | ||||
| 8 | VAL | VAL | LEU | GLU | CYS | TYR | VAL | ARG | LYS | ASP | ||||
| 9 | LEU | VAL | TYR | THR | LYS | ALA | ASN | PRO | THR | PHE | ||||
| 10 | HIS | HIS | TRP | LYS | VAL | ASP | ASN | ARG | LYS | PHE | ||||
| 11 | GLY | LEU | THR | PHE | GLN | SER | PRO | ALA | ASP | ALA | ||||
| 12 | ARG | ALA | PHE | ASP | ARG | GLY | VAL | ARG | LYS | ALA | ||||
| 13 | ILE | GLU | ASP | LEU | ILE | GLU |
Samples:
Sample_1: murine Spred2 EVH1 domain, [U-15N], 1.0 mM; H2O 100%
Sample_2: murine Spred2 EVH1 domain, [U-15N; U-13C], 1.0 mM; H2O 100%
Sample_3: murine Spred2 EVH1 domain, [U-15N; U-13C], 1.0 mM; D2O 100%
Cond_1: pH: 6.0; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N-NOESY-HSQC | not available | not available | Cond_1 |
| 13C-NOESY-HSQC | not available | not available | Cond_1 |
| 13C-HMQC-NOESY | not available | not available | Cond_1 |
| CBCACONNH | not available | not available | Cond_1 |
| CBCANNH | not available | not available | Cond_1 |
| 15N-HSQC | not available | not available | Cond_1 |
| CCCONNH | not available | not available | Cond_1 |
| HCCCONNH | not available | not available | Cond_1 |
| 2D-NOESY | not available | not available | Cond_1 |
| 2D-TOCSY | not available | not available | Cond_1 |
| 2D-COSY | not available | not available | Cond_1 |
| 15N-TOCSY | not available | not available | Cond_1 |
Software:
XWIN-NMR v2.6, Bruker Biospin - collection
ANSIG v3.3, Kraulis - data analysis, chemical shift assignment, peak picking, integration
AZARA v2.1, Boucher - data analysis, peak picking
PSVS v1.3, Hang, Montelione, et al. - refinement, structure validation
X-PLOR_NIH v2.14, Schwieters, Kuszewski, Tjandra and Clore - structure solution, refinement
ARIA v1.2, Linge, O, . - structure solution, refinement
NMR spectrometers:
- Bruker DMX 750 MHz
- Bruker DRX 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAB62849 BAC29425 BAE46587 BAE90243 BAG51727 |
| EMBL | CAH90125 |
| GB | AAH66013 AAI11496 AAI30293 AAI36335 AAP59415 |
| REF | NP_001040559 NP_001121682 NP_001127241 NP_001248029 NP_277058 |
| SP | Q3C2P8 Q5RDN2 Q7Z698 Q924S7 |
| TPG | DAA24561 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts