BMRB Entry 5956
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5956
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Title: NMR solution structure of Max in absence of DNA: Insight into the mechanism of DNA recognition by B-HLH-LZ transcription factors PubMed: 15342239
Deposition date: 2003-09-19 Original release date: 2005-05-03
Authors: Sauve, Simon; Tremblay, Luc; Lavigne, Pierre
Citation: Sauve, S.; Tremblay, L.; Lavigne, P.. "The NMR solution structure of a mutant of the Max b/HLH/LZ free of DNA: insights into the specific and reversible DNA binding mechanism of dimeric transcription factors." J. Mol. Biol. 342, 813-832 (2004).
Assembly members:
Max, polymer, 87 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Max: MADKRAHHNALERKRRDHIK
DSFHSLRDSVPSLQGEKASR
AQILDKATEYIQYMRRKVHT
LQQDIDDLKRQNALLEQQVR
ALEGSGC
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 239 |
| 15N chemical shifts | 75 |
| 1H chemical shifts | 386 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Max*VL subunit 1 | 1 |
| 2 | Max*VL subunit 2 | 1 |
Entities:
Entity 1, Max*VL subunit 1 87 residues - Formula weight is not available
| 1 | MET | ALA | ASP | LYS | ARG | ALA | HIS | HIS | ASN | ALA | ||||
| 2 | LEU | GLU | ARG | LYS | ARG | ARG | ASP | HIS | ILE | LYS | ||||
| 3 | ASP | SER | PHE | HIS | SER | LEU | ARG | ASP | SER | VAL | ||||
| 4 | PRO | SER | LEU | GLN | GLY | GLU | LYS | ALA | SER | ARG | ||||
| 5 | ALA | GLN | ILE | LEU | ASP | LYS | ALA | THR | GLU | TYR | ||||
| 6 | ILE | GLN | TYR | MET | ARG | ARG | LYS | VAL | HIS | THR | ||||
| 7 | LEU | GLN | GLN | ASP | ILE | ASP | ASP | LEU | LYS | ARG | ||||
| 8 | GLN | ASN | ALA | LEU | LEU | GLU | GLN | GLN | VAL | ARG | ||||
| 9 | ALA | LEU | GLU | GLY | SER | GLY | CYS |
Samples:
sample_1: Max, [U-15N], 30 mM
sample_2: Max, [U-13C; U-15N], 30 mM
Expt_condition_1: pH: 6.8; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N HSQC | not available | not available | not available |
| 13C HSQC | not available | not available | not available |
| 15N NOESY HSQC | not available | not available | not available |
| 13C NOESY HSQC | not available | not available | not available |
| 13C aromatic NOESY HSQC | not available | not available | not available |
| HNCA | not available | not available | not available |
| HN(CO)CA | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HCCH TOCSY | not available | not available | not available |
| HCCH COSY | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Varian UNITY-INOVA 600 MHz
Related Database Links:
| PDB |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts