BMRB Entry 5962

Name: Chemical shifts assignments of domain 5 of the ai5gamma group II intron
Published: 2004-03-07

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5962

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Chemical shifts assignments of domain 5 of the ai5gamma group II intron PubMed: 14745440

Deposition date: 2003-10-01 Original release date: 2004-03-07

Authors: Sigel, Roland; Sashital, Dipali; Abramowitz, Dana; Palmer, Arthur; Butcher, Samuel; Pyle, Anna Marie

Citation: Sigel, Roland; Sashital, Dipali; Abramowitz, Dana; Palmer, Arthur; Butcher, Samuel; Pyle, Anna Marie. "Solution structure of domain 5 of a group II intron ribozyme reveals a new RNA motif" Nat. Struct. Mol. Biol. 11, 187-192 (2004).

Assembly members:
Domain 5, polymer, 36 residues, 12297 Da.

Natural source: Common Name: Baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Fungi

Experimental source: Production method: cell free synthesis

Entity Sequences (FASTA):
Domain 5: GGAGCCGUAUGCGAUGAAAG UCGCACGUACGGUUCC

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
13C chemical shifts	97
15N chemical shifts	41
1H chemical shifts	272

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	wildtype domain 5 hairpin	1

Entities:

Entity 1, wildtype domain 5 hairpin 36 residues - 12297 Da.

1

G

A

G

C

G

U

A

U

2

G

C

G

A

U

G

A

G

3

U

C

G

C

A

C

G

U

A

C

4

G

U

C

Samples:

sample_1: Domain 5, [U-13C; U-15N], 0.4 – 0.7 mM; KCl 100 mM; Edta 10 micM

cond_1: ionic strength: 0.11 mM; pH: 6.7; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H,1H NOESY	sample_1	not available	cond_1
3D-13C separated NOESY	sample_1	not available	cond_1
2D 1H-13C HSQC	sample_1	not available	cond_1
2D 1H-1H TOCSY	sample_1	not available	cond_1
3D 1H-13C-1H HCCH TOCSY	sample_1	not available	cond_1
3D 1H-13C-1H HCCH COSY	sample_1	not available	cond_1
2D 2JHN HNN-COSY	sample_1	not available	cond_1

Software:

xwinnmr v2.6 - processing

SPARKY v3 - data analysis

NMR spectrometers:

Bruker Avance 600 MHz
Bruker DMX 500 MHz
Bruker DMX 750 MHz