BMRB Entry 5964
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5964
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Title: Solution structures of the C-terminal headpiece subdomains of human villin and advillin, evaluation of headpiece F-actin binding requirements PubMed: 15096633
Deposition date: 2003-10-06 Original release date: 2008-07-16
Authors: Vermeulen, Wim
Citation: Vermeulen, Wim; Vanhaesebrouck, P.; Van Troys, M.; Verschueren, M.; Fant, F.; Goethals, M.; Ampe, C.; Martins, J.C.; Borremans, F.A.. "Solution structures of the C-terminal headpiece subdomains of human villin and advillin, evaluation of headpiece F-actin-binding requirements " Protein Sci. 13, 1276-1287 (2004).
Assembly members:
human villin C-terminal headpiece, polymer, 36 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
human villin C-terminal headpiece: XLSIEDFTQAFGMTPAAFSA
LPRWKQQNLKKEKGLF
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 223 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | human villin HP35 | 1 |
Entities:
Entity 1, human villin HP35 36 residues - Formula weight is not available
| 1 | ACE | LEU | SER | ILE | GLU | ASP | PHE | THR | GLN | ALA | ||||
| 2 | PHE | GLY | MET | THR | PRO | ALA | ALA | PHE | SER | ALA | ||||
| 3 | LEU | PRO | ARG | TRP | LYS | GLN | GLN | ASN | LEU | LYS | ||||
| 4 | LYS | GLU | LYS | GLY | LEU | PHE |
Samples:
sample_1: human villin C-terminal headpiece 2.0 mM
cond_1: pH: 4.2; temperature: 294 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-1H NOESY | sample_1 | not available | cond_1 |
| 1H-1H TOCSY | sample_1 | not available | cond_1 |
| 1H-1H DQF-COSY | sample_1 | not available | cond_1 |
Software:
Pronto v19990506 - assignment
NMR spectrometers:
- Bruker Avance 500 MHz
Related Database Links:
| BMRB | 5965 |
| PDB | |
| DBJ | BAD97118 BAG36454 BAG62590 |
| EMBL | CAA28355 CAA31386 |
| GB | AAY14886 EAW70620 EHH21671 EHH55165 |
| REF | NP_009058 XP_001090524 XP_001157250 XP_002812896 XP_003272406 |
| SP | P09327 |