BMRB Entry 5972
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5972
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Title: Proton Chemical Shifts for E coli ProP 468-497 peptide PubMed: 14643666
Deposition date: 2003-10-14 Original release date: 2003-12-19
Authors: Zoetewey, David; Tripet, Brian; Kutateladze, Tatiana; Overduin, Michael; Wood, Janet; Hodges, Robert
Citation: Zoetewey, David; Tripet, Brian; Kutateladze, Tatiana; Overduin, Michael; Wood, Janet; Hodges, Robert. "Solution Structure of the C-terminal Antiparallel Coiled-coil Domain of Escherichia Coli Osmosensor ProP " J. Mol. Biol. 334, 1063-1076 (2003).
Assembly members:
ProP, polymer, 36 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
ProP: XXCGGDNIEQKIDDIDHEIA
DLQAKRTRLVQQHPRX
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 214 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ProP subunit A, ProP 468-497 monomer peptide | 1 |
| 2 | ProP subunit B, ProP 468-497 monomer peptide | 1 |
Entities:
Entity 1, ProP subunit A, ProP 468-497 monomer peptide 36 residues - Formula weight is not available
| 1 | ACE | CAM | CYS | GLY | GLY | ASP | ASN | ILE | GLU | GLN | ||||
| 2 | LYS | ILE | ASP | ASP | ILE | ASP | HIS | GLU | ILE | ALA | ||||
| 3 | ASP | LEU | GLN | ALA | LYS | ARG | THR | ARG | LEU | VAL | ||||
| 4 | GLN | GLN | HIS | PRO | ARG | NH2 |
Samples:
sample_1: ProP 3.0 mM; potassium phosphate 50 mM; KCl 100 mM; D2O 10%; H2O 90%
H2O_condition: pH: 5.0; temperature: 298 K; ionic strength: 0.15 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NOESY | sample_1 | not available | H2O_condition |
| TOCSY | sample_1 | not available | H2O_condition |
| COSY | sample_1 | not available | H2O_condition |
Software:
NMRPipe - FT, phasing
CNS v1.1 - simulated annealing
NMRView v5.0.4 - Semi-automated Peakpicking
NMR spectrometers:
- Varian Oxford 600 MHz
- Varian Oxford 500 MHz