BMRB Entry 5978
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5978
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Title: Structure and function of the membrane domain of hepatitis C virus nonstructural protein 5A PubMed: 15247283
Deposition date: 2003-10-20 Original release date: 2004-07-30
Authors: Ramboarina, Stephanie; Brass, Volker; Krieger, Nicole; Montserret, Roland; Ficheux, Damien; Blum, Hubert; Bartenschlager, Ralf; Penin, Francois; Moradpour, Darius
Citation: Penin, Francois; Brass, Volker; Appel, Nicole; Ramboarina, Stephanie; Montserret, Roland; Ficheux, Damien; Blum, Hubert; Bartenschlager, Ralf; Moradpour, Darius. "Structure and function of the membrane anchor domain of hepatitis C virus nonstructural protein 5A " J. Biol. Chem. 279, 40835-40843 (2004).
Assembly members:
HCV NS5A N-terminal, polymer, 31 residues, 3764 Da.
Natural source: Common Name: Hepatitis C virus Taxonomy ID: 11103 Superkingdom: Viruses Kingdom: not available Genus/species: Hepacivirus Hepatitis C virus
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
HCV NS5A N-terminal: SGSWLRDIWDWICEVLSDFK
TWLKAKLMPQL
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 718 |
| 13C chemical shifts | 221 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | NS5A, 1-31 | 1 |
Entities:
Entity 1, NS5A, 1-31 31 residues - 3764 Da.
| 1 | SER | GLY | SER | TRP | LEU | ARG | ASP | ILE | TRP | ASP | ||||
| 2 | TRP | ILE | CYS | GLU | VAL | LEU | SER | ASP | PHE | LYS | ||||
| 3 | THR | TRP | LEU | LYS | ALA | LYS | LEU | MET | PRO | GLN | ||||
| 4 | LEU |
Samples:
Sample_tfe: HCV NS5A N-terminal 1.2 mM; DTT, [U-2H], 10 mM; trifluoroethanol-d2 (CF3CD2OH) 50 % v/v; H2O 50 % v/v
Sample_sds: HCV NS5A N-terminal 1.2 mM; DTT, [U-2H], 10 mM; sodium dodecyl sulfate, [U-2H], 100 mM
Sample_dpc: HCV NS5A N-terminal 1.2 mM; DTT, [U-2H], 10 mM; dodecylphosphocholine, [U-2H], 100 mM
condition_tfe: pH: 4.5; temperature: 293 K
condition_sds: pH: 6.0; temperature: 313 K
condition_dpc: pH: 6.0; temperature: 313 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| DQF-COSY | not available | not available | not available |
| NOESY | not available | not available | not available |
| TOCSY | not available | not available | not available |
| 1H-13C HSQC | not available | not available | not available |
Software:
VNMR v6.1 - processing, assigments
NMR spectrometers:
- Varian UnityPlus 500 MHz