BMRB Entry 5987
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5987
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Title: Interaction of the TAZ1 domain of CREB-binding protein with the activation domain of CITED2: Regulation by competition between intrinsically unstructured ligands for non-identical binding sites PubMed: 14594809
Deposition date: 2003-10-28 Original release date: 2004-02-13
Authors: De Guzman, Roberto; Martinez-Yamout, Maria; Dyson, H. Jane; Wright, Peter
Citation: De Guzman, Roberto; Martinez-Yamout, Maria; Dyson, H. Jane; Wright, Peter. "Interaction of the TAZ1 domain of the CREB-binding protein with the activation domain of CITED2: Regulation by competition between intrinsically unstructured ligands for non-identical binding sites" J. Biol. Chem. 279, 3042-3049 (2004).
Assembly members:
CITED2 C-terminal activation domain, polymer, 51 residues, Formula weight is not available
CBP TAZ1 domain, polymer, 100 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
CITED2 C-terminal activation domain: MTDFIDEEVLMSLVIEMGLD
RIKELPELWLGQNEFDFMTD
FVCKQQPSRVS
CBP TAZ1 domain: ATGPTADPEKRKLIQQQLVL
LLHAHKCQRREQANGEVRAC
SLPHCRTMKNVLNHMTHCQA
GKACQVAHCASSRQIISHWK
NCTRHDCPVCLPLKNASDKR
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 923 |
| 13C chemical shifts | 481 |
| 15N chemical shifts | 145 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CITED2 CAD | 1 |
| 2 | CBP TAZ1 | 2 |
| 3 | ZINC (II) ION, 1 | 3 |
| 4 | ZINC (II) ION, 2 | 3 |
| 5 | ZINC (II) ION, 3 | 3 |
Entities:
Entity 1, CITED2 CAD 51 residues - Formula weight is not available
| 1 | MET | THR | ASP | PHE | ILE | ASP | GLU | GLU | VAL | LEU | ||||
| 2 | MET | SER | LEU | VAL | ILE | GLU | MET | GLY | LEU | ASP | ||||
| 3 | ARG | ILE | LYS | GLU | LEU | PRO | GLU | LEU | TRP | LEU | ||||
| 4 | GLY | GLN | ASN | GLU | PHE | ASP | PHE | MET | THR | ASP | ||||
| 5 | PHE | VAL | CYS | LYS | GLN | GLN | PRO | SER | ARG | VAL | ||||
| 6 | SER |
Entity 2, CBP TAZ1 100 residues - Formula weight is not available
| 1 | ALA | THR | GLY | PRO | THR | ALA | ASP | PRO | GLU | LYS | |
| 2 | ARG | LYS | LEU | ILE | GLN | GLN | GLN | LEU | VAL | LEU | |
| 3 | LEU | LEU | HIS | ALA | HIS | LYS | CYS | GLN | ARG | ARG | |
| 4 | GLU | GLN | ALA | ASN | GLY | GLU | VAL | ARG | ALA | CYS | |
| 5 | SER | LEU | PRO | HIS | CYS | ARG | THR | MET | LYS | ASN | |
| 6 | VAL | LEU | ASN | HIS | MET | THR | HIS | CYS | GLN | ALA | |
| 7 | GLY | LYS | ALA | CYS | GLN | VAL | ALA | HIS | CYS | ALA | |
| 8 | SER | SER | ARG | GLN | ILE | ILE | SER | HIS | TRP | LYS | |
| 9 | ASN | CYS | THR | ARG | HIS | ASP | CYS | PRO | VAL | CYS | |
| 10 | LEU | PRO | LEU | LYS | ASN | ALA | SER | ASP | LYS | ARG |
Entity 3, ZINC (II) ION, 1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: CITED2 C-terminal activation domain, [U-13C; U-15N], 0.5 mM; CBP TAZ1 domain 0.5 mM; Tris-d11 10 mM; NaN3 2 mM; D2O 10%; H2O 90%
sample_2: CITED2 C-terminal activation domain 0.5 mM; CBP TAZ1 domain, [U-13C; U-15N], 0.5 mM; Tris-d11 10 mM; NaN3 2 mM; D2O 10%; H2O 90%
cond_1: pH: 6.8; temperature: 298 K; ionic strength: 16 mM
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCA | not available | not available | cond_1 |
| 3D CBCACONH | not available | not available | cond_1 |
| 3D HNCACB | not available | not available | cond_1 |
| 3D HCCH-TOCSY | not available | not available | cond_1 |
| 3D HCCH-COSY | not available | not available | cond_1 |
| 3D 1H-15N NOESY | not available | not available | cond_1 |
| 3D 1H-13C NOESY | not available | not available | cond_1 |
| 2D HSQC | not available | not available | cond_1 |
| 2D 1H-13C HMQC | not available | not available | cond_1 |
Software:
NMRPipe -
NMR spectrometers:
- BRUKER AVANCE 900 MHz
- BRUKER AVANCE 800 MHz
- BRUKER DRX 600 MHz
Related Database Links:
| SWISS-PROT | P45481 Q92793 |
| REF | NP_596872 XP_001095225 NP_001073315 NP_004371 NP_001020603 |
| PRF | 1923401A |
| GenBank | AAC51770 AAH72594 AAC08447 AAC51331 AAB28651 |
| DBJ | BAG65526 BAE06125 |
| PDB | |
| BMRB | 5327 6268 |
Download simulated HSQC data in one of the following formats:
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