BMRB Entry 5989
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5989
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Title: Cytotoxin I from the venom of the Central Asian cobra Naja Oxiana, in aqueous solution PubMed: 15584897
Deposition date: 2003-10-31 Original release date: 2005-05-20
Authors: Dubinnyi, Maxim
Citation: Dubovskii, P.; Lesovoy, D.; Dubinnyi, Maxim; Konshina, A.; Utkin, Yuri; Efremov, R.; Arseniev, Alexander. "Interaction of three-finger toxins with phospholipid membranes: comparison of S- and P-type cytotoxins" Biochem. J. 387, 807-815 (2005).
Assembly members:
Cytotocin I, polymer, 60 residues, 6821 Da.
Natural source: Common Name: Central Asian Cobra Taxonomy ID: 8657 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Naja Naja oxiana
Experimental source: Production method: purified from the natural source
Entity Sequences (FASTA):
Cytotocin I: LKCNKLVPIAYKTCPEGKNL
CYKMFMMSDLTIPVKRGCID
VCPKNSLLVKYVCCNTDRCN
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 1H chemical shifts | 842 |
| coupling constants | 107 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CTI major form | 1 |
| 2 | CTI minor form | 1 |
Entities:
Entity 1, CTI major form 60 residues - 6821 Da.
| 1 | LEU | LYS | CYS | ASN | LYS | LEU | VAL | PRO | ILE | ALA | |
| 2 | TYR | LYS | THR | CYS | PRO | GLU | GLY | LYS | ASN | LEU | |
| 3 | CYS | TYR | LYS | MET | PHE | MET | MET | SER | ASP | LEU | |
| 4 | THR | ILE | PRO | VAL | LYS | ARG | GLY | CYS | ILE | ASP | |
| 5 | VAL | CYS | PRO | LYS | ASN | SER | LEU | LEU | VAL | LYS | |
| 6 | TYR | VAL | CYS | CYS | ASN | THR | ASP | ARG | CYS | ASN |
Samples:
sample_1: Cytotocin I 3.0 mM; KCl 0.05 M
condition_1: pH: 6.0; temperature: 303 K; ionic strength: 0.05 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| DQF-COSY | sample_1 | not available | condition_1 |
| TOCSY | sample_1 | not available | condition_1 |
| NOESY | sample_1 | not available | condition_1 |
Software:
XWINNMR v3.1a - spectral data processing
XEASY v1.2.11 - peak picking, sequence-specific assignment, manual NOE assignment
CYANA v1.0.6 - automated NOE assignment, structure calculation
NMR spectrometers:
- Bruker DRX 500 MHz