BMRB Entry 6054
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6054
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Title: 1H, 13C and 15N resonance assignments of the methylated N-terminal 16kDa domain of Escherichia coli Ada Protein PubMed: 15213462
Deposition date: 2003-12-18 Original release date: 2008-07-16
Authors: Takinowaki, Hiroto; Matsuda, Yasuhiro; Yoshida, Takuya; Kobayashi, Yuji; Ohkubo, Tadayasu
Citation: Takinowaki, Hiroto; Matsuda, Yasuhiro; Yoshida, Takuya; Kobayashi, Yuji; Ohkubo, Tadayasu. "Letter to the Editer: 1H, 13C and 15N resonance assignments of the N-terminal 16 kDa domain of Escherichia coli Ada Protein" J. Biomol. NMR 29, 447-448 (2004).
Assembly members:
Escherichia coli Ada Protein, polymer, 146 residues, Formula weight is not available
ZINC ION, non-polymer, 65.409 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Escherichia coli Ada Protein: MKKATCLTDDQRWQSVLARD
PNADGEFVFAVRTTGIFXRP
SCRARHALRENVSFYANASE
ALAAGFRPCKRCQPEKANAQ
QHRLDKITHACRLLEQETPV
TLEALADQVAMSPFHLHRLF
KATTGMTPKAWQQAWRARRL
RESLAK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 602 |
| 15N chemical shifts | 147 |
| 1H chemical shifts | 968 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | meC38 N-Ada16k | 1 |
| 2 | ZINC (II) ION | 2 |
Entities:
Entity 1, meC38 N-Ada16k 146 residues - Formula weight is not available
| 1 | MET | LYS | LYS | ALA | THR | CYS | LEU | THR | ASP | ASP | ||||
| 2 | GLN | ARG | TRP | GLN | SER | VAL | LEU | ALA | ARG | ASP | ||||
| 3 | PRO | ASN | ALA | ASP | GLY | GLU | PHE | VAL | PHE | ALA | ||||
| 4 | VAL | ARG | THR | THR | GLY | ILE | PHE | SMC | ARG | PRO | ||||
| 5 | SER | CYS | ARG | ALA | ARG | HIS | ALA | LEU | ARG | GLU | ||||
| 6 | ASN | VAL | SER | PHE | TYR | ALA | ASN | ALA | SER | GLU | ||||
| 7 | ALA | LEU | ALA | ALA | GLY | PHE | ARG | PRO | CYS | LYS | ||||
| 8 | ARG | CYS | GLN | PRO | GLU | LYS | ALA | ASN | ALA | GLN | ||||
| 9 | GLN | HIS | ARG | LEU | ASP | LYS | ILE | THR | HIS | ALA | ||||
| 10 | CYS | ARG | LEU | LEU | GLU | GLN | GLU | THR | PRO | VAL | ||||
| 11 | THR | LEU | GLU | ALA | LEU | ALA | ASP | GLN | VAL | ALA | ||||
| 12 | MET | SER | PRO | PHE | HIS | LEU | HIS | ARG | LEU | PHE | ||||
| 13 | LYS | ALA | THR | THR | GLY | MET | THR | PRO | LYS | ALA | ||||
| 14 | TRP | GLN | GLN | ALA | TRP | ARG | ALA | ARG | ARG | LEU | ||||
| 15 | ARG | GLU | SER | LEU | ALA | LYS |
Entity 2, ZINC (II) ION - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: Escherichia coli Ada Protein, [U-98% 13C; U-98% 15N], 1.0 mM
Ex-cond_1: pH: 6.5; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | sample_1 | not available | Ex-cond_1 |
| HNCO | sample_1 | not available | Ex-cond_1 |
| HNCA | sample_1 | not available | Ex-cond_1 |
| HN(CO)CA | sample_1 | not available | Ex-cond_1 |
| HNCACB | sample_1 | not available | Ex-cond_1 |
| CBCA(CO)NH | sample_1 | not available | Ex-cond_1 |
Software:
No software information available
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| BMRB | 6605 6053 |
| PDB | |
| DBJ | BAA15996.1 BAB36525.1 |
| GenBank | AAA16408.1 AAC75273.1 AAA23412.1 EAY49314.1 AAG57348.1 |
| REF | AP_002809.1 NP_416717.1 ZP_00736701.1 ZP_01698335.1 NP_288793.1 |
| SWISS-PROT | P06134 |
Download simulated HSQC data in one of the following formats:
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