BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules 		Member of WWPDB

BMRB Entry 6073

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6073

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Title: A functional SOD-like protein in Bacillus subtilis   PubMed: 15897454

Deposition date: 2004-01-13 Original release date: 2005-08-18

Authors: Banci, L.; Bertini, I.; Cramaro, F.; Del Conte, R.; Fantoni, A.; Quattrone, A.; Viezzoli, M. Silvia

Citation: Banci, L.; Bertini, I.; Calderone, V.; Cramaro, F.; Del Conte, R.; Fantoni, A.; Mangani, S.; Quattrone, A.; Viezzoli, M.. "A prokaryotic superoxide dismutase paralog lacking two Cu ligands: From largely unstructured in solution to ordered in the crystal"  Proc. Natl. Acad. Sci. U. S. A. 102, 7541-7546 (2005).

Assembly members:
Hypothetical superoxide dismutase-like protein, polymer, 162 residues, Formula weight is not available

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Hypothetical superoxide dismutase-like protein: VVETSAFGHHVQLVNREGKA VGFIEIKESDDEGLDIHISA NSLRPGASLGFHIYEKGSCV RPDFESAGGPFNPLNKEHGF NNPMGHHAGDLPNLEVGADG KVDVIMNAPDTSLKKGSKLN ILDEDGSAFIIHEQADDYLT NPSGNSGARIVCGALLGNNE KQ

Data sets:
Data typeCount
1H chemical shifts868
13C chemical shifts588
15N chemical shifts152

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Hypothetical superoxide dismutase-like protein1

Entities:

Entity 1, Hypothetical superoxide dismutase-like protein 162 residues - Formula weight is not available

1   VALVALGLUTHRSERALAPHEGLYHISHIS
2   VALGLNLEUVALASNARGGLUGLYLYSALA
3   VALGLYPHEILEGLUILELYSGLUSERASP
4   ASPGLUGLYLEUASPILEHISILESERALA
5   ASNSERLEUARGPROGLYALASERLEUGLY
6   PHEHISILETYRGLULYSGLYSERCYSVAL
7   ARGPROASPPHEGLUSERALAGLYGLYPRO
8   PHEASNPROLEUASNLYSGLUHISGLYPHE
9   ASNASNPROMETGLYHISHISALAGLYASP
10   LEUPROASNLEUGLUVALGLYALAASPGLY
11   LYSVALASPVALILEMETASNALAPROASP
12   THRSERLEULYSLYSGLYSERLYSLEUASN
13   ILELEUASPGLUASPGLYSERALAPHEILE
14   ILEHISGLUGLNALAASPASPTYRLEUTHR
15   ASNPROSERGLYASNSERGLYALAARGILE
16   VALCYSGLYALALEULEUGLYASNASNGLU
17   LYSGLN

Samples:

sample_1: Hypothetical superoxide dismutase-like protein, [U-15N], 1.5 mM; H2O 90%; D2O 10%

sample_2: Hypothetical superoxide dismutase-like protein, [U-15N; U-13C], 1.5 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 7.5; temperature: 298 K; pressure: 1 atm

sample_cond_2: pH: 7.5; temperature: 284 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
15N-HSQCnot availablenot availablenot available
15N-CLEANEXnot availablenot availablenot available
HNHAnot availablenot availablenot available
HNHBnot availablenot availablenot available
15N-HSQC-NOESYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
13C-HSQC-NOESYnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
CBCANHnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
HNCOnot availablenot availablenot available
13C H(C)CH-TOCSYnot availablenot availablenot available
13C-(H)CCH-TOCSYnot availablenot availablenot available
13C-CC(CO)NH-TOCSYnot availablenot availablenot available

Software:

CALIBA - structure solution

DYANA - structure solution

AMBER - refinement

XWINNMR - collection

PROCHECK - data analysis

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 600 MHz
  • Bruker Avance 700 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAI85608 BAM52604 BAM58179 GAK81228
EMBL CAB13832 CCU58617 CEI57142 CEJ77567
GB AAC17861 ADV92843 AEP91136 AFI28656 AFQ57880
REF NP_389822 WP_004399243 WP_014477080 WP_014664276 WP_015251895
SP O31851

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts