BMRB Entry 6096
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6096
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Title: The solution structure of the pH-induced monomer of dynein light chain LC8 from Drosophila PubMed: 14767079
Deposition date: 2004-02-09 Original release date: 2004-05-15
Authors: Makokha, M.; Huang, Y.; Montelione, G.; Edison, A.; Barbar, E.
Citation: Makokha, M.; Huang, Y.; Montelione, G.; Edison, A.; Barbar, E.. "The solution structure of the pH-induced monomer of dynein light-chain LC8 from Drosophila " Protein Sci. 13, 727-734 (2004).
Assembly members:
dynein light chain, polymer, 89 residues, 10743 Da.
Natural source: Common Name: Fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
dynein light chain: MSDRKAVIKNADMSEEMQQD
AVDCATQALEKYNIEKDIAA
YIKKEFDKKYNPTWHCIVGR
NFGSYVTHETRHFIYFYLGQ
VAILLFKSG
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 616 |
| 13C chemical shifts | 316 |
| 15N chemical shifts | 88 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | LC8 monomer | 1 |
Entities:
Entity 1, LC8 monomer 89 residues - 10743 Da.
| 1 | MET | SER | ASP | ARG | LYS | ALA | VAL | ILE | LYS | ASN | ||||
| 2 | ALA | ASP | MET | SER | GLU | GLU | MET | GLN | GLN | ASP | ||||
| 3 | ALA | VAL | ASP | CYS | ALA | THR | GLN | ALA | LEU | GLU | ||||
| 4 | LYS | TYR | ASN | ILE | GLU | LYS | ASP | ILE | ALA | ALA | ||||
| 5 | TYR | ILE | LYS | LYS | GLU | PHE | ASP | LYS | LYS | TYR | ||||
| 6 | ASN | PRO | THR | TRP | HIS | CYS | ILE | VAL | GLY | ARG | ||||
| 7 | ASN | PHE | GLY | SER | TYR | VAL | THR | HIS | GLU | THR | ||||
| 8 | ARG | HIS | PHE | ILE | TYR | PHE | TYR | LEU | GLY | GLN | ||||
| 9 | VAL | ALA | ILE | LEU | LEU | PHE | LYS | SER | GLY |
Samples:
sample_1: dynein light chain, [U-15N; U-13C], 0.8 – 1.4 mM; NaCl 50 mM
sample_cond_1: pH: 3.0; temperature: 303 K; ionic strength: 50 mM; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-NOESY | not available | not available | not available |
| 13C-NOESY | not available | not available | not available |
| H/D EXCHANGE | not available | not available | not available |
| HNHA | not available | not available | not available |
| 2D TOCSY | not available | not available | not available |
| NOESY | not available | not available | not available |
| COSY | not available | not available | not available |
| CT-HSQC | not available | not available | not available |
| backbone TR experiments | not available | not available | not available |
| 3D TOCSYS | not available | not available | not available |
Software:
FELIX v97 - processing
NMRPipe v2.1 - processing
AutoAssign v1.9 - data analysis
AutoStructure v1.1.2 - refinement
DYANA v1.5 - refinement
NMR spectrometers:
- Varian INOVA 500 MHz
- Varian INOVA 750 MHz
- Bruker AVANCE 600 MHz
Related Database Links:
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts