BMRB Entry 6127
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6127
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Title: Solution structure of the E. coli bacteriophage P1 encoded HOT protein: a homologue of the theta subunit of E. coli DNA polymerase III PubMed: 15576035
Deposition date: 2004-03-03 Original release date: 2005-02-15
Authors: DeRose, E.; Kirby, T.; Mueller, G.; Chikova, A.; Schaaper, R.; London, R.
Citation: DeRose, E.; Kirby, T.; Mueller, G.; Chikova, A.; Schaaper, R.; London, R.. "Phage like it HOT: solution structure of the bacteriophage P1-encoded HOT protein, a homolog of the theta subunit of E. coli DNA polymerase III" Structure 12, 2221-2231 (2004).
Assembly members:
p1 encoded protein, polymer, 83 residues, Formula weight is not available
Natural source: Common Name: E. coli bacteriophage P1 Taxonomy ID: not available Superkingdom: Bacteriophage Kingdom: not available Genus/species: not available not available
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
p1 encoded protein: MYDWNIAAKSQEERDKVNVD
LAASGVAYKERLNIPVIAEQ
VAREQPENLRTYFMERLRHY
RQLSLQLPKGSDPAYQKDDA
VKK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 341 |
| 1H chemical shifts | 466 |
| 15N chemical shifts | 87 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HOT protein | 1 |
Entities:
Entity 1, HOT protein 83 residues - Formula weight is not available
| 1 | MET | TYR | ASP | TRP | ASN | ILE | ALA | ALA | LYS | SER | ||||
| 2 | GLN | GLU | GLU | ARG | ASP | LYS | VAL | ASN | VAL | ASP | ||||
| 3 | LEU | ALA | ALA | SER | GLY | VAL | ALA | TYR | LYS | GLU | ||||
| 4 | ARG | LEU | ASN | ILE | PRO | VAL | ILE | ALA | GLU | GLN | ||||
| 5 | VAL | ALA | ARG | GLU | GLN | PRO | GLU | ASN | LEU | ARG | ||||
| 6 | THR | TYR | PHE | MET | GLU | ARG | LEU | ARG | HIS | TYR | ||||
| 7 | ARG | GLN | LEU | SER | LEU | GLN | LEU | PRO | LYS | GLY | ||||
| 8 | SER | ASP | PRO | ALA | TYR | GLN | LYS | ASP | ASP | ALA | ||||
| 9 | VAL | LYS | LYS |
Samples:
sample_1: p1 encoded protein, [U-13C; U-15N], 1.3 mM; Tris 5 mM; NaCl 100 mM; NaN3 5 mM; H2O 90%; D2O 10%
sample_cond_1: pH: 7; temperature: 298 K; ionic strength: 100 mM; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | not available | sample_cond_1 |
| 3D 15N-separated NOESY | sample_1 | not available | sample_cond_1 |
Software:
NMRPipe v2.1 Rev 2002.044.17.08 - processing
NMRView v5.0.4 - data analysis
ARIA v1.2 - structure solution
CNS v1.1 - structure solution, refinement
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| PDB | |
| GB | AAQ14071 AAQ14179 EHN41319 EHU11765 EII43590 |
| REF | WP_001286531 WP_001667237 WP_024224114 YP_006567 |
Download simulated HSQC data in one of the following formats:
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SPARKY: Backbone
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