BMRB Entry 6156
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6156
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Title: 1H Assigned Chemical Shifts for FMBP-1 tandem repeat 1 in 30% (v/v) TFE and water solution
Deposition date: 2004-03-22 Original release date: 2004-06-30
Authors: Kawaguchi, Kyosuke; Yamaki, Takeshi; Aizawa, Tomoyasu; Takiya, Shigeharu; Demura, Makoto; Nitta, Katsutoshi
Citation: Kawaguchi, Kyosuke; Yamaki, Takeshi; Aizawa, Tomoyasu; Takiya, Shigeharu; Demura, Makoto; Nitta, Katsutoshi. "1H Assigned Chemical Shifts for FMBP-1 tandem repeat 1 in 30% (v/v) TFE and water solution" The BMRB entry is the only known published source for the data..
Assembly members:
fibroin-modulator-binding-protein-1, polymer, 23 residues, 2538 Da.
Natural source: Common Name: Silkworm Taxonomy ID: 7091 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bombyx mori
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
fibroin-modulator-binding-protein-1: ETSEERAARLAKMSAYAAQR
LAN
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 296 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | FMBP-1 | 1 |
Entities:
Entity 1, FMBP-1 23 residues - 2538 Da.
| 1 | GLU | THR | SER | GLU | GLU | ARG | ALA | ALA | ARG | LEU | ||||
| 2 | ALA | LYS | MET | SER | ALA | TYR | ALA | ALA | GLN | ARG | ||||
| 3 | LEU | ALA | ASN |
Samples:
sample_1: fibroin-modulator-binding-protein-1 1.5 mM; TFE 30 % (v/v)
sample_2: fibroin-modulator-binding-protein-1 1.5 mM
cond_1: pH: 4.6; temperature: 293 K
cond_2: pH: 4.0; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| DQF-COSY | not available | not available | not available |
| TOCSY | not available | not available | not available |
| NOESY | not available | not available | not available |
Software:
NMRPipe - raw spectral data processing
XEASY - assignments, peak picking
NMR spectrometers:
- Bruker DRX 600 MHz
- Jeol ALPHA 600 MHz
Related Database Links:
| PDB |