BMRB Entry 6194
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6194
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Title: 1H, 13C, and 15N Chemical Shift Assignments for Omega-atracotoxin-Hv1a at pH 6.0
Deposition date: 2004-04-26 Original release date: 2004-06-25
Authors: Tedford, Hugo; Maciejewski, Mark; King, Glenn
Citation: Tedford, Hugo; Maciejewski, Mark; King, Glenn. "1H, 13C, and 15N Chemical Shift Assignments for Omega-atracotoxin-Hv1a at pH 6.0" . ., .-..
Assembly members:
Omega-atracotoxin-Hv1a, polymer, 37 residues, Formula weight is not available
Natural source: Common Name: Blue Mountains Funnel-Web Spider Taxonomy ID: 6904 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Hadronyche versuta
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Omega-atracotoxin-Hv1a: SPTCIPSGQPCPYNENCCSQ
SCTFKENENGNTVKRCD
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 130 |
| 15N chemical shifts | 40 |
| 1H chemical shifts | 218 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Omega-ACTX-Hv1a | 1 |
Entities:
Entity 1, Omega-ACTX-Hv1a 37 residues - Formula weight is not available
| 1 | SER | PRO | THR | CYS | ILE | PRO | SER | GLY | GLN | PRO | ||||
| 2 | CYS | PRO | TYR | ASN | GLU | ASN | CYS | CYS | SER | GLN | ||||
| 3 | SER | CYS | THR | PHE | LYS | GLU | ASN | GLU | ASN | GLY | ||||
| 4 | ASN | THR | VAL | LYS | ARG | CYS | ASP |
Samples:
sample_1: Omega-atracotoxin-Hv1a, [U-13C; U-15N], 2 – 5 mM; NaCl 50 mM; sodium phosphate 20 mM
sample_2: Omega-atracotoxin-Hv1a, [U-13C; U-15N], 2 – 5 mM; D2O 100%
Ex-cond_1: ionic strength: 0.075 M; pH: 6.0; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-15N NOESY-HSQC | not available | not available | Ex-cond_1 |
| 3D 1H-13C HSQC-NOESY | not available | not available | Ex-cond_1 |
| 3D HNCACB | not available | not available | Ex-cond_1 |
| 3D HNCO | not available | not available | Ex-cond_1 |
| 3D HNCACO | not available | not available | Ex-cond_1 |
| 3D HCCH-COSY | not available | not available | Ex-cond_1 |
| 3D (H)CCOHN-TOCSY | not available | not available | Ex-cond_1 |
| 3D 1H-15N TOCSY | not available | not available | Ex-cond_1 |
| 3D HNHA | not available | not available | Ex-cond_1 |
| 3D HNHB | not available | not available | Ex-cond_1 |
| 2D 1H-1H NOESY | not available | not available | Ex-cond_1 |
Software:
nmrPipe - spectral processing
Xeasy - resonance assignment
NMR spectrometers:
- Varian INOVA 500 MHz
- Varian INOVA 600 MHz
Related Database Links:
| BMRB | 4233 4937 5702 6192 |
| PDB | |
| EMBL | CAI79354 |
| GenBank | ABP63656 |
| SWISS-PROT | P56207 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts