BMRB Entry 6226
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6226
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Title: 1H, 13C, and 15N Chemical Shift Assignments for the C-terminal domain of EW29 PubMed: 15756471
Deposition date: 2004-06-07 Original release date: 2004-12-16
Authors: Hemmi, Hikaru
Citation: Hemmi, Hikaru; Kuno, Atsushi; Ito, Shigeyasu; Suzuki, Ryuichiro; Kaneko, Satoshi; Hasegawa, Tsunemi; Hirabayashi, Jun; Kasai, Ken-ichi. "Letter to the Editor: 1H, 13C and 15N chemical shift assignment of the C-terminal 15 kDa domain of novel galactose-binding protein from the earth worm Lumbricus terrestris" J. Biomol. NMR 30, 377-378 (2004).
Assembly members:
C-terminal domain of EW29, polymer, 132 residues, Formula weight is not available
Natural source: Common Name: common earthworm Taxonomy ID: 6398 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Lumbricus terrestris
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
C-terminal domain of EW29: MKPKFFYIKSELNGKVLDIE
GQNPAPGSKIITWDQKKGPT
AVNQLWYTDQQGVIRSKLND
FAIDASHEQIETQPFDPNNP
KRAWIVSGNTIAQLSDRDIV
LDIIKSDKEAGAHICAWKQH
GGPNQKFIIESE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 591 |
| 15N chemical shifts | 133 |
| 1H chemical shifts | 911 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | EW29 monomer | 1 |
Entities:
Entity 1, EW29 monomer 132 residues - Formula weight is not available
| 1 | MET | LYS | PRO | LYS | PHE | PHE | TYR | ILE | LYS | SER | ||||
| 2 | GLU | LEU | ASN | GLY | LYS | VAL | LEU | ASP | ILE | GLU | ||||
| 3 | GLY | GLN | ASN | PRO | ALA | PRO | GLY | SER | LYS | ILE | ||||
| 4 | ILE | THR | TRP | ASP | GLN | LYS | LYS | GLY | PRO | THR | ||||
| 5 | ALA | VAL | ASN | GLN | LEU | TRP | TYR | THR | ASP | GLN | ||||
| 6 | GLN | GLY | VAL | ILE | ARG | SER | LYS | LEU | ASN | ASP | ||||
| 7 | PHE | ALA | ILE | ASP | ALA | SER | HIS | GLU | GLN | ILE | ||||
| 8 | GLU | THR | GLN | PRO | PHE | ASP | PRO | ASN | ASN | PRO | ||||
| 9 | LYS | ARG | ALA | TRP | ILE | VAL | SER | GLY | ASN | THR | ||||
| 10 | ILE | ALA | GLN | LEU | SER | ASP | ARG | ASP | ILE | VAL | ||||
| 11 | LEU | ASP | ILE | ILE | LYS | SER | ASP | LYS | GLU | ALA | ||||
| 12 | GLY | ALA | HIS | ILE | CYS | ALA | TRP | LYS | GLN | HIS | ||||
| 13 | GLY | GLY | PRO | ASN | GLN | LYS | PHE | ILE | ILE | GLU | ||||
| 14 | SER | GLU |
Samples:
sample_1: C-terminal domain of EW29, [U-15N; U-13C], 0.9 mM
Ex-cond_1: pH: 6.1; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | sample_1 | not available | Ex-cond_1 |
Software:
xwinnmr v3.5 - acquisiton
FELIX v2000 - processing
SPARKY v3.100 - Peak assignment
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| PDB |
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