BMRB Entry 6244
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR6244
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Title: Novel all alpha-helical fold for gene target gi3844938 from Mycoplasma genitalium
Deposition date: 2004-06-22 Original release date: 2004-08-04
Authors: Shi, Jianxia; Pelton, Jeffrey; Wemmer, David
Citation: Shi, Jianxia; Pelton, Jeffrey; Yokota, Hisao; Kim, Rosalind; Wemmer, David. "Novel all alpha-helical fold for gene target gi3844938 from Mycoplasma genitalium " . ., .-..
Assembly members:
gi3844938, polymer, 137 residues, 15703 Da.
Natural source: Common Name: Mycoplasma genitalium Taxonomy ID: 243273 Superkingdom: Eubacteria Kingdom: not available Genus/species: Mycoplasma genitalium
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
gi3844938: MEQNNIKEQLISFFNQACST
HQERLDFICSTRESDTFSSV
DVPLEPIKNIIEITKDENQQ
IEITKIAVNNIKTLSSVGAT
GQYMASFFSTNSEPAIIFCV
IYFLYHFGFLKDNNKKQIIK
KAYETIADNIADYLNEN
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 812 |
| 13C chemical shifts | 557 |
| 15N chemical shifts | 132 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | gi3844938 | 1 |
Entities:
Entity 1, gi3844938 137 residues - 15703 Da.
| 1 | MET | GLU | GLN | ASN | ASN | ILE | LYS | GLU | GLN | LEU | ||||
| 2 | ILE | SER | PHE | PHE | ASN | GLN | ALA | CYS | SER | THR | ||||
| 3 | HIS | GLN | GLU | ARG | LEU | ASP | PHE | ILE | CYS | SER | ||||
| 4 | THR | ARG | GLU | SER | ASP | THR | PHE | SER | SER | VAL | ||||
| 5 | ASP | VAL | PRO | LEU | GLU | PRO | ILE | LYS | ASN | ILE | ||||
| 6 | ILE | GLU | ILE | THR | LYS | ASP | GLU | ASN | GLN | GLN | ||||
| 7 | ILE | GLU | ILE | THR | LYS | ILE | ALA | VAL | ASN | ASN | ||||
| 8 | ILE | LYS | THR | LEU | SER | SER | VAL | GLY | ALA | THR | ||||
| 9 | GLY | GLN | TYR | MET | ALA | SER | PHE | PHE | SER | THR | ||||
| 10 | ASN | SER | GLU | PRO | ALA | ILE | ILE | PHE | CYS | VAL | ||||
| 11 | ILE | TYR | PHE | LEU | TYR | HIS | PHE | GLY | PHE | LEU | ||||
| 12 | LYS | ASP | ASN | ASN | LYS | LYS | GLN | ILE | ILE | LYS | ||||
| 13 | LYS | ALA | TYR | GLU | THR | ILE | ALA | ASP | ASN | ILE | ||||
| 14 | ALA | ASP | TYR | LEU | ASN | GLU | ASN |
Samples:
sample_1: gi3844938, [U-93% 13C; U-98% 15N], 1.0 mM; KPO4 25 mM; NaCl 100 mM; EDTA 1 mM; TCEP 1 mM
Ex-cond_1: pH: 6.7 na; temperature: 298 K; ionic strength: 0.14 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N-HSQC | not available | not available | not available |
| HNCA | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HNCACB | not available | not available | not available |
| HNCACO | not available | not available | not available |
| HACANH | not available | not available | not available |
| hC CONH | not available | not available | not available |
| Hc CONH | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
| 15N-NOESY-HSQC | not available | not available | not available |
| 15N-TOCSY-HSQC | not available | not available | not available |
| 2D NOESY | not available | not available | not available |
| 2D TOCSY | not available | not available | not available |
| 2D 13C Double half-filtered TOCSY | not available | not available | not available |
| 2D 13C Dboule half-filtered NOESY | not available | not available | not available |
Software:
NMRView v5.0.4 - Data analysis
nmrPipe v2.1 - Data processing
NMR spectrometers:
- DRX Bruker 500 MHz
- DRX Bruker 600 MHz
Related Database Links:
| PDB | |
| GB | AAC71579 ABY79523 AFQ03197 AFQ03682 AFQ04187 |
| REF | NP_073025 WP_009885812 YP_006599905 YP_006600390 YP_006600895 |
| SP | P47596 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts