BMRB Entry 6299
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6299
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Title: Structure of a Chordin-like Cysteine-rich Repeat (VWC module) from Collagen IIA PubMed: 15466413
Deposition date: 2004-08-26 Original release date: 2004-12-06
Authors: O'Leary, J.; Hamilton, J.; Deane, C.; Valeyev, N.; Sandell, L.; Downing, A.
Citation: O'Leary, J.; Hamilton, J.; Deane, C.; Valeyev, N.; Sandell, L.; Downing, A.. "Solution Structure and Dynamics of a Prototypical Chordin-like Cysteine-rich Repeat (von Willebrand Factor Type C Module) from Collagen IIA" J. Biol. Chem. 279, 53857-53866 (2004).
Assembly members:
alpha 1 type II collagen isoform 1, polymer, 73 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Pichia pastoris
Entity Sequences (FASTA):
alpha 1 type II collagen isoform 1: YVEFQEAGSCVQDGQRYNDK
DVWKPEPCRICVCDTGTVLC
DDIICEDVKDCLSPEIPFGE
CCPICPADLAAAA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 177 |
| 15N chemical shifts | 65 |
| 1H chemical shifts | 414 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | alpha 1 type II collagen isoform 1 | 1 |
Entities:
Entity 1, alpha 1 type II collagen isoform 1 73 residues - Formula weight is not available
| 1 | TYR | VAL | GLU | PHE | GLN | GLU | ALA | GLY | SER | CYS | ||||
| 2 | VAL | GLN | ASP | GLY | GLN | ARG | TYR | ASN | ASP | LYS | ||||
| 3 | ASP | VAL | TRP | LYS | PRO | GLU | PRO | CYS | ARG | ILE | ||||
| 4 | CYS | VAL | CYS | ASP | THR | GLY | THR | VAL | LEU | CYS | ||||
| 5 | ASP | ASP | ILE | ILE | CYS | GLU | ASP | VAL | LYS | ASP | ||||
| 6 | CYS | LEU | SER | PRO | GLU | ILE | PRO | PHE | GLY | GLU | ||||
| 7 | CYS | CYS | PRO | ILE | CYS | PRO | ALA | ASP | LEU | ALA | ||||
| 8 | ALA | ALA | ALA |
Samples:
sample_1: alpha 1 type II collagen isoform 1, [U-15N], 0.8 mM; phosphate buffer 50 mM; NaN3 0.02%; H2O 90%; D2O 10%
sample_2: alpha 1 type II collagen isoform 1, [U-15N], 0.8 mM; phosphate buffer 50 mM; NaN3 0.02%; D2O 99.9%
sample_3: alpha 1 type II collagen isoform 1, [U-13C; U-15N], 0.8 mM; phosphate buffer 50 mM; NaN3 0.02%; H2O 90%; D2O 10%
sample_cond_1: ionic strength: 50 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | not available | not available | not available |
| HMQC-J | not available | not available | not available |
| 2D NOESY | not available | not available | not available |
Software:
CYANA v1.0.6 - refinement, structure solution
NMR spectrometers:
- GE Omega 750 MHz
Related Database Links:
| PDB |
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