BMRB Entry 6328
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6328
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Title: Assessment of the robustness of a serendipitous zinc-binding fold: mutagenesis and protein grafting PubMed: 15698569
Deposition date: 2004-09-24 Original release date: 2005-03-16
Authors: Sharpe, B.; Liew, C.; Wilce, J.; Crossley, M.; Matthews, J.; Mackay, J.
Citation: Sharpe, B.; Liew, C.; Kwan, A.; Wilce, J.; Crossley, M.; Matthews, J.; Mackay, J.. "Assessment of the robustness of a serendipitous zinc-binding fold: mutagenesis and protein grafting" Structure 13, 257-266 (2005).
Assembly members:
CREB Binding Protein (E.C.2.3.1.48), polymer, 25 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemically synthesized
Entity Sequences (FASTA):
CREB Binding Protein (E.C.2.3.1.48): AVSACALSKCAAAANVAAHM
THCAK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 134 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CREB Binding Protein | 1 |
| 2 | ZINC (II) ION | 2 |
Entities:
Entity 1, CREB Binding Protein 25 residues - Formula weight is not available
| 1 | ALA | VAL | SER | ALA | CYS | ALA | LEU | SER | LYS | CYS | ||||
| 2 | ALA | ALA | ALA | ALA | ASN | VAL | ALA | ALA | HIS | MET | ||||
| 3 | THR | HIS | CYS | ALA | LYS |
Entity 2, ZINC (II) ION - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: CREB Binding Protein (E.C.2.3.1.48) 0.4 mM; TCEP 0.5 mM; ZnSO4 1 mM; D2O 100%
sample_cond_1: pH: 6.9; pressure: 1 atm; temperature: 275 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| DQF-COSY | sample_1 | not available | sample_cond_1 |
| 2D TOCSY | sample_1 | not available | sample_cond_1 |
| 2D NOESY | sample_1 | not available | sample_cond_1 |
| E-COSY | sample_1 | not available | sample_cond_1 |
Software:
xwinnmr v2.5 - processing
XEASY v1.3.13 - data analysis
DYANA v1.5 - data analysis
CNS v1.0 - refinement
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| PDB |