BMRB Entry 6344
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6344
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Title: 1H, 13C, and 15N Chemical Shift Assignments for human protein HSPCO34
Deposition date: 2004-10-08 Original release date: 2010-08-25
Authors: Ramelot, Theresa; Montelione, Guy; Acton, Thomas; Kennedy, Michael
Citation: Ramelot, Theresa; Kennedy, Michael. "Solution NMR Structure of Human protein HSPCO34. Northeast Structural Genomics Target HR1958." The BMRB entry is the only known published source for the data. (2004).
Assembly members:
HR1958, polymer, 153 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
HR1958: MGHHHHHHSHMRKIDLCLSS
EGSEVILATSSDEKHPPENI
IDGNPETFWTTTGMFPQEFI
ICFHKHVRIERLVIQSYFVQ
TLKIEKSTSKEPVDFEQWIE
KDLVHTEGQLQNEEIVAHGS
ATYLRFIIVSAFDHFASVHS
VSAEGTVVSNLSS
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 1013 |
| 13C chemical shifts | 645 |
| 15N chemical shifts | 145 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HR1958 | 1 |
Entities:
Entity 1, HR1958 153 residues - Formula weight is not available
| 1 | MET | GLY | HIS | HIS | HIS | HIS | HIS | HIS | SER | HIS | ||||
| 2 | MET | ARG | LYS | ILE | ASP | LEU | CYS | LEU | SER | SER | ||||
| 3 | GLU | GLY | SER | GLU | VAL | ILE | LEU | ALA | THR | SER | ||||
| 4 | SER | ASP | GLU | LYS | HIS | PRO | PRO | GLU | ASN | ILE | ||||
| 5 | ILE | ASP | GLY | ASN | PRO | GLU | THR | PHE | TRP | THR | ||||
| 6 | THR | THR | GLY | MET | PHE | PRO | GLN | GLU | PHE | ILE | ||||
| 7 | ILE | CYS | PHE | HIS | LYS | HIS | VAL | ARG | ILE | GLU | ||||
| 8 | ARG | LEU | VAL | ILE | GLN | SER | TYR | PHE | VAL | GLN | ||||
| 9 | THR | LEU | LYS | ILE | GLU | LYS | SER | THR | SER | LYS | ||||
| 10 | GLU | PRO | VAL | ASP | PHE | GLU | GLN | TRP | ILE | GLU | ||||
| 11 | LYS | ASP | LEU | VAL | HIS | THR | GLU | GLY | GLN | LEU | ||||
| 12 | GLN | ASN | GLU | GLU | ILE | VAL | ALA | HIS | GLY | SER | ||||
| 13 | ALA | THR | TYR | LEU | ARG | PHE | ILE | ILE | VAL | SER | ||||
| 14 | ALA | PHE | ASP | HIS | PHE | ALA | SER | VAL | HIS | SER | ||||
| 15 | VAL | SER | ALA | GLU | GLY | THR | VAL | VAL | SER | ASN | ||||
| 16 | LEU | SER | SER |
Samples:
sample_1: HR1958, [U-15N; U-13C], 1 mM; Mes 20 mM; NaCl 100 mM; CaCl2 5 mM; DTT 10 mM; NaN3 0.02%
sample_conditions: pH: 6.5; temperature: 298 K; ionic strength: 0.1 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| Data collected on 600mHz Varian Inova spectrometer. | sample_1 | not available | sample_conditions |
| [U-15N, U-13C]-HR1958 | sample_1 | not available | sample_conditions |
| 2D 1H-15N HSQC - regular and NH2only | sample_1 | not available | sample_conditions |
| 2D 1H-13C HSQC -aliph, alpha, arom | sample_1 | not available | sample_conditions |
| 3D HNCO | sample_1 | not available | sample_conditions |
| 3D HNCACB | sample_1 | not available | sample_conditions |
| 3D CBCA(CO)NH | sample_1 | not available | sample_conditions |
| 3D HCCH-COSY | sample_1 | not available | sample_conditions |
| 3D HCCH-TOCSY | sample_1 | not available | sample_conditions |
| 3D H(CC)(CO)NH-TOCSY | sample_1 | not available | sample_conditions |
| 3D (H)CC(CO)NH-TOCSY | sample_1 | not available | sample_conditions |
| [U-15N, 5%-13C]-HR1958 | sample_1 | not available | sample_conditions |
| 1H-13C HSQC to get stereospecific assignements for Leu, Val isopropyl groups. | sample_1 | not available | sample_conditions |
| Data collected on 750mHz Varian Inova spectrometer. | sample_1 | not available | sample_conditions |
| 2D 1H-15N HSQC | sample_1 | not available | sample_conditions |
| 3D HNHA | sample_1 | not available | sample_conditions |
| 3D 15N-edited NOESY-HSQC | sample_1 | not available | sample_conditions |
| 3D 13C-edited NOESY-HSQC -aliph, arom | sample_1 | not available | sample_conditions |
| 4D CC-NOESY (2H2O) | sample_1 | not available | sample_conditions |
Software:
No software information available
NMR spectrometers:
- Varian Inova 600 MHz
- Varian Inova 750 MHz
- Varian Inova 800 MHz
Related Database Links:
| PDB | |
| GB | AAH05245 AAQ03618 AAQ03994 ADQ32445 AIC56497 |
| REF | NP_001244683 NP_057210 XP_001149402 XP_002810844 XP_002810845 |
| SP | Q9Y547 |
Download simulated HSQC data in one of the following formats:
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or all simulated shifts
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