BMRB Entry 6347
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6347
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Title: Evidence for domain-specific recognition of SK and Kv channels by MTX and HsTx1 scorpion toxins PubMed: 15498765
Deposition date: 2004-10-11 Original release date: 2004-10-29
Authors: Regaya, I.; Beeton, C.; Ferrat, G.; Andreotti, N.; Chandy, G.; Darbon, H.; De Waard, M.; Sabatier, J.
Citation: Regaya, I.; Beeton, C.; Ferrat, G.; Andreotti, N.; Darbon, H.; De Waard, M.; Sabatier, J.. "Evidence for domain-specific recognition of SK and Kv channels by MTX and HsTx1 scorpion toxins" J. Biol. Chem. 279, 55690-55696 (2004).
Assembly members:
MTx-HsTx1, polymer, 34 residues, Formula weight is not available
Natural source: Common Name: Asian forest scorpions Taxonomy ID: 118530 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Heterometrus spinifer
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
MTx-HsTx1: VSCTGSKDCYAPCRKQTGCP
YGKCMNRKCKCNRC
- assigned_chemical_shifts
Data type | Count |
1H chemical shifts | 158 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | MTx-HsTx1 | 1 |
Entities:
Entity 1, MTx-HsTx1 34 residues - Formula weight is not available
1 | VAL | SER | CYS | THR | GLY | SER | LYS | ASP | CYS | TYR | ||||
2 | ALA | PRO | CYS | ARG | LYS | GLN | THR | GLY | CYS | PRO | ||||
3 | TYR | GLY | LYS | CYS | MET | ASN | ARG | LYS | CYS | LYS | ||||
4 | CYS | ASN | ARG | CYS |
Samples:
sample_1: MTx-HsTx1 mM
sample_cond_1: pH: 3; temperature: 300 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
DQF-COSY | not available | not available | not available |
2D TOCSY | not available | not available | not available |
2D NOESY | not available | not available | not available |
Software:
xwinnmr v2.0 - processing
XEASY v1.3.13 - data analysis
DIANA v2.8 - structure solution
CNS v1.1 - refinement
NMR spectrometers:
- Bruker DMX 500 MHz
Related Database Links:
PDB |