BMRB Entry 6380
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6380
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Title: 1H, 13C, and 15N Chemical Shift Assignments for pig prion protein PubMed: 15647367
Deposition date: 2004-11-09 Original release date: 2005-01-13
Authors: Schorn, Christian; Lysek, Dominikus; Wuthrich, Kurt
Citation: Lysek, Dominikus; Schorn, Christian; Nivon, Lucas; Esteve-Moya, Vicent; Christen, Barbara; Calzolai, Luigi; von Schroetter, Christine; Fiorito, Francesco; Herrmann, Torsten; Guntert, Peter; Wuthrich, Kurt. "Prion protein NMR structures of cats, dogs, pigs, and sheep" Proc. Natl. Acad. Sci. U.S.A. 102, 640-645 (2005).
Assembly members:
pig prion protein, polymer, 113 residues, Formula weight is not available
Natural source: Common Name: Pig Taxonomy ID: 9823 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Sus scrofa
Experimental source: Production method: recombinant technology Host organism: Eschericia coli
Entity Sequences (FASTA):
pig prion protein: GSVVGGLGGYMLGSAMSRPL
IHFGSDYEDRYYRENMYRYP
NQVYYRPVDQYSNQNSFVHD
CVNITVKQHTVTTTTKGENF
TETDVKMIERVVEQMCITQY
QKEYEAYAQRGAS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 307 |
| 15N chemical shifts | 123 |
| 1H chemical shifts | 716 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | pig prion protein monomer | 1 |
Entities:
Entity 1, pig prion protein monomer 113 residues - Formula weight is not available
| 1 | GLY | SER | VAL | VAL | GLY | GLY | LEU | GLY | GLY | TYR | ||||
| 2 | MET | LEU | GLY | SER | ALA | MET | SER | ARG | PRO | LEU | ||||
| 3 | ILE | HIS | PHE | GLY | SER | ASP | TYR | GLU | ASP | ARG | ||||
| 4 | TYR | TYR | ARG | GLU | ASN | MET | TYR | ARG | TYR | PRO | ||||
| 5 | ASN | GLN | VAL | TYR | TYR | ARG | PRO | VAL | ASP | GLN | ||||
| 6 | TYR | SER | ASN | GLN | ASN | SER | PHE | VAL | HIS | ASP | ||||
| 7 | CYS | VAL | ASN | ILE | THR | VAL | LYS | GLN | HIS | THR | ||||
| 8 | VAL | THR | THR | THR | THR | LYS | GLY | GLU | ASN | PHE | ||||
| 9 | THR | GLU | THR | ASP | VAL | LYS | MET | ILE | GLU | ARG | ||||
| 10 | VAL | VAL | GLU | GLN | MET | CYS | ILE | THR | GLN | TYR | ||||
| 11 | GLN | LYS | GLU | TYR | GLU | ALA | TYR | ALA | GLN | ARG | ||||
| 12 | GLY | ALA | SER |
Samples:
sample_1: pig prion protein, [U-13C; U-15N], 1 mM
conditions_1: pH: 4.5; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCA | sample_1 | not available | conditions_1 |
| 15N edited NOESY | sample_1 | not available | conditions_1 |
| 13C edited NOESY | sample_1 | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Bruker DRX 750 MHz
Related Database Links:
| PDB | |
| GB | AAA92862 AAV83492 AAV83493 AAV83494 AAV83495 |
| REF | NP_001008687 |
| SP | P49927 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
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