BMRB Entry 6393
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6393
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Title: Refined solution structure of the human TSG-6 Link module PubMed: 15718240
Deposition date: 2004-11-17 Original release date: 2005-04-12
Authors: Blundell, C.; Teriete, P.; Kahmann, J.; Pickford, A.; Campbell, I.
Citation: Blundell, C.; Almond, A.; Mahoney, D.; Deangelis, P.; Campbell, I.; Day, A.. "Towards a structure for a hyaluronan-TSG-6 complex by modeling and NMR spectroscopy: Insights into other members of the link module superfamily" J. Biol. Chem. ., .-..
Assembly members:
human TSG-6, polymer, 98 residues, Formula weight is not available
HA octasaccharide, polymer, 8 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
human TSG-6: GVYHREARSGKYKLTYAEAK
AVCEFEGGHLATYKQLEAAR
KIGFHVCAAGWMAKGRVGYP
IVKPGPNCGFGKTGIIDYGI
RLNRSERWDAYCYNPHAK
HA octasaccharide: XXXXXXXX
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 681 |
| 13C chemical shifts | 427 |
| 15N chemical shifts | 113 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Link TSG6 | 1 |
| 2 | HA octasaccharide | 2 |
Entities:
Entity 1, Link TSG6 98 residues - Formula weight is not available
| 1 | GLY | VAL | TYR | HIS | ARG | GLU | ALA | ARG | SER | GLY | ||||
| 2 | LYS | TYR | LYS | LEU | THR | TYR | ALA | GLU | ALA | LYS | ||||
| 3 | ALA | VAL | CYS | GLU | PHE | GLU | GLY | GLY | HIS | LEU | ||||
| 4 | ALA | THR | TYR | LYS | GLN | LEU | GLU | ALA | ALA | ARG | ||||
| 5 | LYS | ILE | GLY | PHE | HIS | VAL | CYS | ALA | ALA | GLY | ||||
| 6 | TRP | MET | ALA | LYS | GLY | ARG | VAL | GLY | TYR | PRO | ||||
| 7 | ILE | VAL | LYS | PRO | GLY | PRO | ASN | CYS | GLY | PHE | ||||
| 8 | GLY | LYS | THR | GLY | ILE | ILE | ASP | TYR | GLY | ILE | ||||
| 9 | ARG | LEU | ASN | ARG | SER | GLU | ARG | TRP | ASP | ALA | ||||
| 10 | TYR | CYS | TYR | ASN | PRO | HIS | ALA | LYS |
Entity 2, HA octasaccharide 8 residues - Formula weight is not available
| 1 | BNA | BGC | BNA | BGC | BNA | BGC | BNA | BGC |
Samples:
sample_1: human TSG-6 1.0 mM; HA octasaccharide 1.0 mM; Na ions 2 mM; D2O 100%
sample_2: human TSG-6, [U-15N], 2.0 mM; HA octasaccharide 1.0 mM; Na ions 2 mM
sample_3: human TSG-6, [U-15N; U-13C], 2.8 mM; HA octasaccharide 1.0 mM; Na ions 2 mM
sample_cond_1: pH: 6.0; temperature: 298 K; ionic strength: 2 mM; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N-NOESY-HSQC | not available | not available | not available |
| 15N-HSQC-NOESY-HSQC | not available | not available | not available |
| 13C-NOESY-HSQC | not available | not available | not available |
| 2D-NOESY | not available | not available | not available |
Software:
FELIX v2.3 - spectrum processing
XEasy - spectrum analysis
NMR spectrometers:
- n/a n/a 750 MHz
- n/a n/a 750 MHz
Related Database Links:
| BMRB | 6392 7221 7222 |
| PDB | |
| DBJ | BAE24204 BAJ20799 |
| EMBL | CAD12353 CAD13434 CAH74219 |
| GB | AAA03342 AAB00792 AAC53527 AAH21155 AAH30205 |
| REF | NP_001007814 NP_001075375 NP_001075780 NP_001252869 NP_009046 |
| SP | O08859 P98065 P98066 |
| TPG | DAA32671 |
Download simulated HSQC data in one of the following formats:
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SPARKY: Backbone
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