BMRB Entry 6480
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR6480
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: The loss of stability of a naturally occurring ATP7A mutant is the cause of Menkes disease PubMed: 16083905
Deposition date: 2005-02-02 Original release date: 2005-11-14
Authors: Banci, L.; Bertini, I.; Cantini, F.; Migliardi, M.; Rosato, A.; Wang, S.
Citation: Banci, L.; Bertini, I.; Cantini, F.; Migliardi, M.; Rosato, A.; Wang, S.. "An Atomic-level Investigation of the Disease-causing A629P Mutant of the Menkes Protein, ATP7A." J. Mol. Biol. 352, 409-417 (2005).
Assembly members:
Copper-transporting ATPase 1(E.C.3.6.3.4), polymer, 75 residues, Formula weight is not available
CU1, non-polymer, 63.546 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Copper-transporting ATPase 1(E.C.3.6.3.4): MGDGVLELVVRGMTCASCVH
KIESSLTKHRGILYCSVALA
TNKAHIKYDPEIIGPRDIIH
TIESLGFEASLVKIE
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 70 |
| 1H chemical shifts | 469 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Copper-transporting ATPase 1 | 1 |
| 2 | COPPER (I) ION | 2 |
Entities:
Entity 1, Copper-transporting ATPase 1 75 residues - Formula weight is not available
| 1 | MET | GLY | ASP | GLY | VAL | LEU | GLU | LEU | VAL | VAL | ||||
| 2 | ARG | GLY | MET | THR | CYS | ALA | SER | CYS | VAL | HIS | ||||
| 3 | LYS | ILE | GLU | SER | SER | LEU | THR | LYS | HIS | ARG | ||||
| 4 | GLY | ILE | LEU | TYR | CYS | SER | VAL | ALA | LEU | ALA | ||||
| 5 | THR | ASN | LYS | ALA | HIS | ILE | LYS | TYR | ASP | PRO | ||||
| 6 | GLU | ILE | ILE | GLY | PRO | ARG | ASP | ILE | ILE | HIS | ||||
| 7 | THR | ILE | GLU | SER | LEU | GLY | PHE | GLU | ALA | SER | ||||
| 8 | LEU | VAL | LYS | ILE | GLU |
Entity 2, COPPER (I) ION - Cu - 63.546 Da.
| 1 | CU1 |
Samples:
sample_1: Copper-transporting ATPase 1(E.C.3.6.3.4), [U-15N], 0.8 mM; DTT 5 mM; phosphate buffer 100 mM; H2O 90%; D2O 10%
sample_2: Copper-transporting ATPase 1(E.C.3.6.3.4), [U-15N; U-13C], 0.8 mM; DTT 5 mM; phosphate buffer 100 mM; H2O 90%; D2O 10%
sample_3: Copper-transporting ATPase 1(E.C.3.6.3.4) 1.0 mM; DTT 5 mM; phosphate buffer 100 mM; H2O 90%; D2O 10%
sample_cond_1: ionic strength: 100 mM; pH: 7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | not available | not available | sample_cond_1 |
| CBCANH | not available | not available | sample_cond_1 |
| CBCACONH | not available | not available | sample_cond_1 |
| HNCO | not available | not available | sample_cond_1 |
| HNCACO | not available | not available | sample_cond_1 |
| 2D NOESY | not available | not available | sample_cond_1 |
| 2D TOCSY | not available | not available | sample_cond_1 |
| HNHA | not available | not available | sample_cond_1 |
Software:
xwinnmr - collection
CARA v1.2 - data analysis
DYANA v1.5 - structure solution
AMBER v5.0 - refinement
NMR spectrometers:
- Bruker AVANCE 500 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts