BMRB Entry 6489
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6489
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Title: Chemical shift assignments for subunit c of the F1Fo ATP Synthase from Bacillus PS3 PubMed: 16497328
Deposition date: 2005-02-04 Original release date: 2006-04-24
Authors: Nakano, Takayuki; Ikegami, Takahisa; Suzuki, Toshiharu; Yoshida, Masasuke; Akutsu, Hideo
Citation: Nakano, Takayuki; Ikegami, Takahisa; Suzuki, Toshiharu; Yoshida, Masasuke; Akutsu, Hideo. "A New Solution Structure of ATP Synthase Subunit c from Thermophilic Bacillus PS3, Suggesting a Local Conformational Change for H(+)-Translocation" J. Mol. Biol. ., .-. (2006).
Assembly members:
F1Fo-ATP synthase subunit c, polymer, 72 residues, Formula weight is not available
Natural source: Common Name: Thermophilic Bacillus PS3 Taxonomy ID: 2334 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus Bacillus sp. PS3
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
F1Fo-ATP synthase subunit c: MSLGVLAAAIAVGLGALGAG
IGNGLIVSRTIEGIARQPEL
RPVLQTTMFIGVALVEALPI
IGVVFSFIYLGR
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 508 |
| 13C chemical shifts | 306 |
| 15N chemical shifts | 67 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | F1Fo-c | 1 |
Entities:
Entity 1, F1Fo-c 72 residues - Formula weight is not available
| 1 | MET | SER | LEU | GLY | VAL | LEU | ALA | ALA | ALA | ILE | ||||
| 2 | ALA | VAL | GLY | LEU | GLY | ALA | LEU | GLY | ALA | GLY | ||||
| 3 | ILE | GLY | ASN | GLY | LEU | ILE | VAL | SER | ARG | THR | ||||
| 4 | ILE | GLU | GLY | ILE | ALA | ARG | GLN | PRO | GLU | LEU | ||||
| 5 | ARG | PRO | VAL | LEU | GLN | THR | THR | MET | PHE | ILE | ||||
| 6 | GLY | VAL | ALA | LEU | VAL | GLU | ALA | LEU | PRO | ILE | ||||
| 7 | ILE | GLY | VAL | VAL | PHE | SER | PHE | ILE | TYR | LEU | ||||
| 8 | GLY | ARG |
Samples:
sample_1: F1Fo-ATP synthase subunit c 2 mM; CDCl3, [U-2D], 75%; CD3OH, [U-2D], 25%
sample_2: F1Fo-ATP synthase subunit c, [U-15N], 2 mM; CDCl3, [U-2D], 75%; CD3OH, [U-2D], 25%
sample_3: F1Fo-ATP synthase subunit c, [U-13C; U-15N], 2 mM; CDCl3, [U-2D], 75%; CD3OH, [U-2D], 25%
condition_1: pH: 2; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N-1N-HSQC | sample_2 | not available | condition_1 |
| 2D_NOESY | sample_1 | not available | condition_1 |
| CBCA(CO)NH | sample_3 | not available | condition_1 |
| CBCANH | sample_3 | not available | condition_1 |
| HNCO | sample_3 | not available | condition_1 |
| HN(CA)CO | sample_3 | not available | condition_1 |
| HBHANH | sample_3 | not available | condition_1 |
| HBHA(CO)NH | sample_3 | not available | condition_1 |
| H(CCO)NH | sample_3 | not available | condition_1 |
| C(CO)NH | sample_3 | not available | condition_1 |
Software:
NMRPipe v2.2 - processing
Sparky v3.106 - data analysis
NMR spectrometers:
- Bruker DRX 800 MHz
- Bruker DRX 600 MHz
- Bruker DRX 500 MHz
Related Database Links:
| BMRB | 11550 |
| PDB | |
| DBJ | BAA07244 BAA07251 BAD77648 GAD12918 GAJ57176 |
| EMBL | CAA30649 |
| GB | ACX79968 ADI28261 ADU95796 AEV21076 AGE23948 |
| PIR | LWHWA3 |
| REF | WP_011232830 WP_014196950 YP_003254450 YP_003672838 YP_004133939 |
| SP | P00845 P41015 P42011 Q5KUI8 |
Download simulated HSQC data in one of the following formats:
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SPARKY: Backbone
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