BMRB Entry 6510
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6510
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Title: Structure and inter-domain interactions of domain II from the blood stage malarial protein, apical membrane antigen 1 PubMed: 15964019
Deposition date: 2005-02-17 Original release date: 2005-08-22
Authors: Feng, Zhi-Ping; Keizer, David; Stevenson, Rachel; Yao, Shenggen; Babon, Jeffrey; Murphy, Vince; Anders, Robin; Norton, Raymond
Citation: Feng, Zhi-Ping; Keizer, David; Stevenson, Rachel; Yao, Shenggen; Babon, Jeffrey; Murphy, Vince; Anders, Robin; Norton, Raymond. "Structure and Inter-domain Interactions of Domain II from the Blood-stage Malarial Protein, Apical Membrane Antigen 1 " J. Mol. Biol. 350, 641-656 (2005).
Assembly members:
Apical membrane antigen ! Domain II, polymer, 140 residues, Formula weight is not available
Natural source: Common Name: Plasmodium falciparum Taxonomy ID: 5833 Superkingdom: Eukaryota Kingdom: not available Genus/species: Plasmodium falciparum
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Apical membrane antigen ! Domain II: MRGSHHHHHHGSNAKFGLWV
DGNCEDIPHVNEFPAIDLFE
CNKLVFELSASDQPKQYEQH
LTDYEKIKEGFKNKNASMIK
SAFLPTGAFKADRYKSHGKG
YNWGNYNTETQKCEIFNVKP
TCLINNSSYIATTALSHPIE
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 702 |
| 13C chemical shifts | 483 |
| 15N chemical shifts | 140 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | AMA1 DII | 1 |
Entities:
Entity 1, AMA1 DII 140 residues - Formula weight is not available
| 1 | MET | ARG | GLY | SER | HIS | HIS | HIS | HIS | HIS | HIS | |
| 2 | GLY | SER | ASN | ALA | LYS | PHE | GLY | LEU | TRP | VAL | |
| 3 | ASP | GLY | ASN | CYS | GLU | ASP | ILE | PRO | HIS | VAL | |
| 4 | ASN | GLU | PHE | PRO | ALA | ILE | ASP | LEU | PHE | GLU | |
| 5 | CYS | ASN | LYS | LEU | VAL | PHE | GLU | LEU | SER | ALA | |
| 6 | SER | ASP | GLN | PRO | LYS | GLN | TYR | GLU | GLN | HIS | |
| 7 | LEU | THR | ASP | TYR | GLU | LYS | ILE | LYS | GLU | GLY | |
| 8 | PHE | LYS | ASN | LYS | ASN | ALA | SER | MET | ILE | LYS | |
| 9 | SER | ALA | PHE | LEU | PRO | THR | GLY | ALA | PHE | LYS | |
| 10 | ALA | ASP | ARG | TYR | LYS | SER | HIS | GLY | LYS | GLY | |
| 11 | TYR | ASN | TRP | GLY | ASN | TYR | ASN | THR | GLU | THR | |
| 12 | GLN | LYS | CYS | GLU | ILE | PHE | ASN | VAL | LYS | PRO | |
| 13 | THR | CYS | LEU | ILE | ASN | ASN | SER | SER | TYR | ILE | |
| 14 | ALA | THR | THR | ALA | LEU | SER | HIS | PRO | ILE | GLU |
Samples:
sample_1: Apical membrane antigen ! Domain II, [U-95% 13C; U-90% 15N], 0.1 – 0.8 mM
Ex-cond_1: pH: 4.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
xwinnmr v3.5 - Data collection, processing
xeasy v1,3 - Data analysis
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker Avance 500 MHz
- Bruker Avance 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAM85358 BAM85364 BAM85367 BAM85373 BAM85379 |
| EMBL | CAB97181 CAB97184 CAB97187 CAB97189 CAB97193 |
| GB | AAA29475 AAA29476 AAA29719 AAB36701 AAB50405 |
| REF | XP_001348015 |
| SP | P22621 P50490 |
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